Format

Send to

Choose Destination
Brain Struct Funct. 2018 Jan;223(1):391-414. doi: 10.1007/s00429-017-1501-4. Epub 2017 Aug 29.

Neuronal connections of the central amygdalar nucleus with refeeding-activated brain areas in rats.

Author information

1
Department of Endocrine Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, 43 Szigony St, Budapest, 1083, Hungary.
2
Neuroendocrinology Program, Semmelweis University Neurosciences Doctoral School, Budapest, Hungary.
3
Department of Neuroscience, Faculty of Information Technology, Pázmány Péter Catholic University, Budapest, Hungary.
4
Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, Tupper Research Institute, Tufts Medical Center, Boston, MA, USA.
5
Department of Neuroscience, Tufts University School of Medicine, Boston, MA, USA.
6
Department of Endocrine Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, 43 Szigony St, Budapest, 1083, Hungary. fekete.csaba@koki.mta.hu.
7
Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, Tupper Research Institute, Tufts Medical Center, Boston, MA, USA. fekete.csaba@koki.mta.hu.

Abstract

Following fasting, satiety is accompanied by neuronal activation in brain areas including the central amygdalar nucleus (CEA). Since CEA is known to inhibit food intake, we hypothesized that CEA contributes to the termination of meal during refeeding. To better understand the organization of this satiety-related circuit, the interconnections of the CEA with refeeding-activated neuronal groups were elucidated using retrograde (cholera toxin-β subunit, CTB) and anterograde (phaseolus vulgaris leucoagglutinin, PHA-L) tracers in male rats. C-Fos-immunoreactivity was used as marker of neuronal activation. The refeeding-activated input of the CEA primarily originated from the paraventricular thalamic, parasubthalamic and parabrachial nuclei. Few CTB-c-Fos double-labeled neurons were detected in the prefrontal cortex, lateral hypothalamic area, nucleus of the solitary tract (NTS) and the bed nuclei of the stria terminalis (BNST). Only few refeeding-activated proopiomelanocortin-producing neurons of the arcuate nucleus projected to the CEA. Anterograde tract tracing revealed a high density of PHAL-labeled axons contacted with refeeding-activated neurons in the BNST, lateral hypothalamic area, parasubthalamic, paraventricular thalamic and parabrachial nuclei and NTS; a low density of labeled axons was found in the paraventricular hypothalamic nucleus. Chemogenetic activation of the medial CEA (CEAm) inhibited food intake during the first hour of refeeding, while activation of lateral CEA had no effect. These data demonstrate the existence of reciprocal connections between the CEA and distinct refeeding-activated hypothalamic, thalamic and brainstem nuclei, suggesting the importance of short feedback loops in the regulation of satiety and importance of the CEAm in the regulation of food intake during refeeding.

KEYWORDS:

Anterograde tract tracing; Connectivity map; Parabrachial nucleus; Parasubthalamic nucleus; Retrograde tract tracing; Satiety; c-Fos

PMID:
28852859
PMCID:
PMC5773374
DOI:
10.1007/s00429-017-1501-4
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center