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Development. 2017 Oct 1;144(19):3590-3601. doi: 10.1242/dev.147967. Epub 2017 Aug 29.

Smooth muscle cell recruitment to lymphatic vessels requires PDGFB and impacts vessel size but not identity.

Author information

1
Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm, Sweden.
2
Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala, Sweden.
3
Integrated Cardio Metabolic Centre (ICMC), Karolinska Institutet, Novum, Blickagången 6, SE14157 Huddinge, Sweden.
4
Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala, Sweden taija.makinen@igp.uu.se lars.jakobsson@ki.se.
5
Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm, Sweden taija.makinen@igp.uu.se lars.jakobsson@ki.se.

Abstract

Tissue fluid drains through blind-ended lymphatic capillaries, via smooth muscle cell (SMC)-covered collecting vessels into venous circulation. Both defective SMC recruitment to collecting vessels and ectopic recruitment to lymphatic capillaries are thought to contribute to vessel failure, leading to lymphedema. However, mechanisms controlling lymphatic SMC recruitment and its role in vessel maturation are unknown. Here, we demonstrate that platelet-derived growth factor B (PDGFB) regulates lymphatic SMC recruitment in multiple vascular beds. PDGFB is selectively expressed by lymphatic endothelial cells (LECs) of collecting vessels. LEC-specific deletion of Pdgfb prevented SMC recruitment causing dilation and failure of pulsatile contraction of collecting vessels. However, vessel remodelling and identity were unaffected. Unexpectedly, Pdgfb overexpression in LECs did not induce SMC recruitment to capillaries. This was explained by the demonstrated requirement of PDGFB extracellular matrix (ECM) retention for lymphatic SMC recruitment, and the low presence of PDGFB-binding ECM components around lymphatic capillaries. These results demonstrate the requirement of LEC-autonomous PDGFB expression and retention for SMC recruitment to lymphatic vessels, and suggest an ECM-controlled checkpoint that prevents SMC investment of capillaries, which is a common feature in lymphedematous skin.

KEYWORDS:

Contraction; Lymphatic vasculature; Lymphedema; Morphogenesis; PDGFB; Smooth muscle cell

PMID:
28851707
PMCID:
PMC5665477
DOI:
10.1242/dev.147967
[Indexed for MEDLINE]
Free PMC Article

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