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Nat Plants. 2017 Sep;3(9):742-748. doi: 10.1038/s41477-017-0005-9. Epub 2017 Aug 28.

Prominent topologically associated domains differentiate global chromatin packing in rice from Arabidopsis.

Author information

1
Department of General Genetics, Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany. chang.liu@zmbp.uni-tuebingen.de.
2
Department of Molecular Biology, Max Planck Institute for Developmental Biology, Spemannstraße 35, 72076, Tübingen, Germany. chang.liu@zmbp.uni-tuebingen.de.
3
National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology (SIPPE), Shanghai Institutes for Biological Sciences (SIBS), 200032, Shanghai, People's Republic of China.
4
University of Chinese Academy of Sciences, 200032, Shanghai, People's Republic of China.
5
Department of Molecular Biology, Max Planck Institute for Developmental Biology, Spemannstraße 35, 72076, Tübingen, Germany. weigel@weigelworld.org.

Abstract

The non-random three-dimensional organization of genomes is critical for many cellular processes. Recently, analyses of genome-wide chromatin packing in the model dicot plant Arabidopsis thaliana have been reported 1-4 . At a kilobase scale, the A. thaliana chromatin interaction network is highly correlated with a range of genomic and epigenomic features 1-4 . Surprisingly, topologically associated domains (TADs), which appear to be a prevalent structural feature of genome packing in many animal species, are not prominent in the A. thaliana genome 1,2,4-6 . Using a genome-wide chromatin conformation capture approach, Hi-C (ref. 7 ), we report high-resolution chromatin packing patterns of another model plant, rice. We unveil new structural features of chromatin organization at both chromosomal and local levels compared to A. thaliana, with thousands of distinct TADs that cover about a quarter of the rice genome. The rice TAD boundaries are associated with euchromatic epigenetic marks and active gene expression, and enriched with a sequence motif that can be recognized by plant-specific TCP proteins. In addition, we report chromosome decondensation in rice seedlings undergoing cold stress, despite local chromatin packing patterns remaining largely unchanged. The substantial variation found already in a comparison of two plant species suggests that chromatin organization in plants might be more diverse than in multicellular animals.

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PMID:
28848243
DOI:
10.1038/s41477-017-0005-9
[Indexed for MEDLINE]

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