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Food Res Int. 2017 Sep;99(Pt 2):959-968. doi: 10.1016/j.foodres.2017.01.017. Epub 2017 Jan 21.

Lycopene rich extract from red guava (Psidium guajava L.) displays anti-inflammatory and antioxidant profile by reducing suggestive hallmarks of acute inflammatory response in mice.

Author information

1
Núcleo de Pesquisa em Biodiversidade e Biotecnologia-BIOTEC, Universidade Federal do Piauí-UFPI, Campus Ministro Reis Velloso-CMRV, Parnaíba, PI, Brazil.
2
Laboratório de Fisio-Farmacologia Experimental-LAFFEX, Universidade Federal do Piauí-UFPI, Campus Ministro Reis Velloso-CMRV, Parnaíba, PI, Brazil.
3
Laboratório de Farmacologia da Inflamação e do Câncer-LAFICA, Universidade Federal do Ceará-UFC, Fortaleza, CE, Brazil.
4
Laboratório de Neurofarmacologia, Universidade Federal do Ceará-UFC, Fortaleza, CE, Brazil.
5
Núcleo de Estudos em Microscopia e Processamento de Imagens-NEMPI, Universidade Federal do Ceará-UFC, Fortaleza, CE, Brazil.
6
Phytobios Nordeste LTDA, Parnaíba, Piauí, Brazil.
7
Área de Morfologia da Faculdade de Medicina, Universidade de Brasília-UnB, Brasília, DF, Brazil.
8
Núcleo de Pesquisa em Biodiversidade e Biotecnologia-BIOTEC, Universidade Federal do Piauí-UFPI, Campus Ministro Reis Velloso-CMRV, Parnaíba, PI, Brazil; Área de Morfologia da Faculdade de Medicina, Universidade de Brasília-UnB, Brasília, DF, Brazil. Electronic address: jrsaleite@gmail.com.

Abstract

This study investigated the anti-inflammatory activity of the extract (LEG) and purified (LPG) lycopene from guava (Psidium guajava L.), as well as some mechanisms possibly involved in this effect. The anti-inflammatory activity was initially assessed using paw edema induced by Carrageenan, Dextran, Compound 48/80, Histamine and Prostaglandin E2 in Swiss mice. A peritonitis model was used to evaluate neutrophil migration, the activity of myeloperoxidase (MPO) and reduced glutathione (GSH) concentration; while the effect on the expression of iNOS, COX-2 and NF-κB, was assessed by immunohistochemistry analysis. Results showed that oral and intraperitoneal administration of LEG and LPG inhibited inflammation caused by carrageenan. LPG (12.5mg/kg p.o.) significantly inhibited the edema formation induced by different phlogistic agents and immunostaining for iNOS, COX-2 and NF-κB. Leukocytes migration in paw tissue and peritoneal cavity was reduced, as well as MPO concentration, whereas GSH levels increased. Thus, lycopene-rich extract from red guava has beneficial effect on acute inflammation, offering protection against the consequences of oxidative stress by downregulating inflammatory mediators and inhibiting gene expression involved in inflammation.

KEYWORDS:

Carotenoids; Inflammation; Natural antioxidant; Psidium guajava L. fruit

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