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Int J Mol Sci. 2017 Aug 28;18(9). pii: E1865. doi: 10.3390/ijms18091865.

Monitoring and Measuring Autophagy.

Author information

1
Focal Area Infection Biology, Biozentrum, University of Basel, Klingelbergstrasse 50/70, 4056 Basel, Switzerland. saori.yoshii@unibas.ch.
2
Department of Biochemistry and Molecular Biology, Graduate School and Faculty of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. nmizu@m.u-tokyo.ac.jp.

Abstract

Autophagy is a cytoplasmic degradation system, which is important for starvation adaptation and cellular quality control. Recent advances in understanding autophagy highlight its importance under physiological and pathological conditions. However, methods for monitoring autophagic activity are complicated and the results are sometimes misinterpreted. Here, we review the methods used to identify autophagic structures, and to measure autophagic flux in cultured cells and animals. We will also describe the existing autophagy reporter mice that are useful for autophagy studies and drug testing. Lastly, we will consider the attempts to monitor autophagy in samples derived from humans.

KEYWORDS:

Keima; LC3; autophagic flux; autophagosome; isolation membrane; p62/SQSTM1

PMID:
28846632
PMCID:
PMC5618514
DOI:
10.3390/ijms18091865
[Indexed for MEDLINE]
Free PMC Article

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