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Mol Cell. 2017 Sep 7;67(5):733-743.e4. doi: 10.1016/j.molcel.2017.07.026. Epub 2017 Aug 24.

Structural and Functional Impacts of ER Coactivator Sequential Recruitment.

Author information

1
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
2
National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
3
Departments of Molecular and Cellular Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
4
Department of Biochemistry and Molecular Biology, University of Texas Houston Medical School, Houston, TX 77030, USA.
5
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA; National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: wah@bcm.edu.
6
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: berto@bcm.edu.

Abstract

Nuclear receptors recruit multiple coactivators sequentially to activate transcription. This "ordered" recruitment allows different coactivator activities to engage the nuclear receptor complex at different steps of transcription. Estrogen receptor (ER) recruits steroid receptor coactivator-3 (SRC-3) primary coactivator and secondary coactivators, p300/CBP and CARM1. CARM1 recruitment lags behind the binding of SRC-3 and p300 to ER. Combining cryo-electron microscopy (cryo-EM) structure analysis and biochemical approaches, we demonstrate that there is a close crosstalk between early- and late-recruited coactivators. The sequential recruitment of CARM1 not only adds a protein arginine methyltransferase activity to the ER-coactivator complex, it also alters the structural organization of the pre-existing ERE/ERα/SRC-3/p300 complex. It induces a p300 conformational change and significantly increases p300 HAT activity on histone H3K18 residues, which, in turn, promotes CARM1 methylation activity on H3R17 residues to enhance transcriptional activity. This study reveals a structural role for a coactivator sequential recruitment and biochemical process in ER-mediated transcription.

KEYWORDS:

CARM1; SRC-3; coactivator; cryo-EM structure; estrogen receptor; histone modification; p300; sequential recruitment

PMID:
28844863
PMCID:
PMC5657569
DOI:
10.1016/j.molcel.2017.07.026
[Indexed for MEDLINE]
Free PMC Article

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