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Biomed Pharmacother. 2017 Nov;95:245-251. doi: 10.1016/j.biopha.2017.08.073. Epub 2017 Sep 12.

Paraquat exposure-induced Parkinson's disease-like symptoms and oxidative stress in Drosophila melanogaster: Neuroprotective effect of Bougainvillea glabra Choisy.

Author information

1
Laboratório de Estudos Físico-Químicos e Produtos Naturais (LEFQPN), Universidade Federal do Pampa, Uruguaiana, RS, Brazil.
2
Grupo Interdisciplinar de Pesquisa em Prática de Ensino (GIPPE), Universidade Federal do Pampa, Uruguaiana, RS, Brazil.
3
Grupo de Estudos em Nutrição, Saúde e Qualidade de Vida (GENSQ), Universidade Federal do Pampa, Uruguaiana, RS, Brazil.
4
Laboratório de Estudos Físico-Químicos e Produtos Naturais (LEFQPN), Universidade Federal do Pampa, Uruguaiana, RS, Brazil. Electronic address: eltondenardin@gmail.com.

Abstract

Extracts from the leaves of Bougainvillea glabra Choisy are used in traditional medicines, but their actions on the central nervous system have not been studied. In the present study, we investigated the potential neuroprotective effects of Bougainvillea glabra Choisy leaf extract (BG extract) against paraquat (PQ)-induced neurotoxicity. Male adult wild-type flies (1- 4days old) were exposed to PQ (3.5mM) and/or BG extract (120μg/mL) through food for 4days. PQ-fed flies had decreased locomotor capacity in negative geotaxis and crossing number assays and had a higher incidence of mortality than the control group. PQ neurotoxicity was also associated with a marked decrease in dopamine levels and increase in acetylcholinesterase (AChE) activity, reactive oxygen species (ROS) production and lipid peroxidation. Co-exposure to BG extract prevented mortality, and dopamine depletion, improved locomotor performance and decreased AChE activity, ROS production and lipid peroxidation. GC-MS and HPLC analyses of BG extract revealed the presence of many antioxidant compounds such as phytol, α,γ-tocopherol, squalene, stigmasterol, geranylgeraniol, quercetin, and caffeic, vanillic, coumaric, ferulic acids. Our results showed neuroprotective effects of BG extract, reflecting the presence of antioxidant compounds. Thus, we suggested that B. glabra leaves could be considered an effective agent in the prevention of neurological disorders, where dopamine depletion and/or oxidative stress are involved, as in Parkinson's disease (PD).

KEYWORDS:

Antioxidants; Bougainvillea glabra choisy; Drosophila melanogaster; Neurotoxicity; Paraquat; Parkinson’s disease like

PMID:
28843913
DOI:
10.1016/j.biopha.2017.08.073
[Indexed for MEDLINE]

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