Visualization of IAV Genomes at the Single-Cell Level

Dan Wang; Wenjun Ma

doi:10.1016/j.tim.2017.08.003

Visualization of IAV Genomes at the Single-Cell Level

Trends Microbiol. 2017 Oct;25(10):781-782. doi: 10.1016/j.tim.2017.08.003. Epub 2017 Aug 23.

Authors

Dan Wang¹, Wenjun Ma²

Affiliations

¹ Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA. Electronic address: dan.wang@sdstate.edu.
² Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506, USA. Electronic address: wma@vet.k-state.edu.

Abstract

Different influenza A viruses (IAVs) infect the same cell in a host, and can subsequently produce new viruses through genome reassortment. By combining padlock probe RNA labeling with a single-cell analysis, a new approach effectively captures IAV genome trafficking and defines a time window for genome reassortment from same-cell coinfections.

Keywords: influenza A virus genomes; single cell; visualization.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Comment

MeSH terms

Genome, Viral / genetics*
Host-Pathogen Interactions / genetics
Humans
Influenza A virus / genetics*
Influenza, Human / virology
Single-Cell Analysis
Virus Replication / genetics

Abstract

Publication types

MeSH terms

Grants and funding