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Methods Mol Biol. 2017;1668:39-60. doi: 10.1007/978-1-4939-7283-8_4.

Targeted Lipidomic Analysis of Myoblasts by GC-MS and LC-MS/MS.

Author information

1
Institut Mondor de Recherche Biomédicale (IMRB), U955-E10 Biologie du Système Neuromusculaire, Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort (EnvA), Maisons-Alfort, France.
2
Department of Cardiology, University of California, San Diego, La Jolla, CA, USA.
3
Plateforme de Lipidomique-uBourgogne, INSERM UMR1231/LabEx LipSTIC, UFR des Sciences de Santé - Bâtiment B3, Dijon, France.
4
Institut Mondor de Recherche Biomédicale (IMRB), U955-E10 Biologie du Système Neuromusculaire, Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort (EnvA), Maisons-Alfort, France. laurent.tiret@vet-alfort.fr.

Abstract

Lipids represent ∼10% of the cell dry mass and play essential roles in membrane composition and physical properties, energy storage, and signaling pathways. In the developing or the regenerating skeletal muscle, modifications in the content or the flipping between leaflets of membrane lipid components can modulate the fusion capacity of myoblasts, thus constituting one of the regulatory mechanisms underlying myofiber growth. Recently, few genes controlling these qualitative and quantitative modifications have started to be unraveled. The precise functional characterization of these genes requires both qualitative and quantitative evaluations of a global lipid profile. Here, we describe a lipidomic protocol using mass spectrometry, allowing assessing the content of fatty acids, glycerophospholipids, and cholesterol in the routinely used C2C12 mouse myoblast cell line, or in primary cultures of mouse myoblasts.

KEYWORDS:

CL; Chromatography; LPC; MUFA; Mass spectrometry; Membrane lipid composition; Myoblast fusion; VLCFA

PMID:
28842901
DOI:
10.1007/978-1-4939-7283-8_4
[Indexed for MEDLINE]

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