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Acta Trop. 2017 Dec;176:236-241. doi: 10.1016/j.actatropica.2017.08.016. Epub 2017 Aug 24.

Leishmania tropica infected human lesions: Whole genome transcription profiling.

Author information

1
Immunotherpy and Leishmania Vaccine Research Dept., Pasteur Institute of Iran, Tehran, Iran.
2
Zurich, Switzerland.
3
Cutaneous Leishmaniasis Research Center, Emam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
4
Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran.
5
Immunotherpy and Leishmania Vaccine Research Dept., Pasteur Institute of Iran, Tehran, Iran. Electronic address: s_rafati@yahoo.com.

Abstract

Leishmania (L.) tropica is the main causative agent of anthroponotic cutaneous leishmaniasis (CL) in Iran. Defining the host inflammatory response in the L. tropica lesions are crucial for the development of new treatment modalities. High-throughput RNA sequencing provides a powerful method for characterization of the human gene expression profile in L. tropica lesions. Comparing the transcription profile of the L. tropica skin lesions with normal skin identified over 5000 differentially regulated genes. Gene set enrichment analysis indicated significant activation of key immunological pathways related to antigen processing and presentation. In addition, we observed a substantial upregulation of immunoglobulin genes in lesion samples, highlighting the remarkable involvement of B cells in the infection site. To our knowledge, this study is the first report to build a comprehensive picture of transcriptome changes in acute human skin lesions during infection by L. tropica.

KEYWORDS:

Cutaneous leishmaniasis; Gene expression profiling; High-throughput RNA sequencing; Leishmania tropica

[Indexed for MEDLINE]

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