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Curr Microbiol. 2017 Dec;74(12):1453-1460. doi: 10.1007/s00284-017-1340-9. Epub 2017 Aug 24.

Antimicrobial and Antioxidant Activities of the Extract and Fractions of Tetradenia riparia (Hochst.) Codd (Lamiaceae) Leaves from Brazil.

Author information

1
Pharmaceutical Sciences, State University of Western Paraná, Cascavel, Paraná, 85.819-110, Brazil.
2
Pharmaceutical Sciences, State University of Maringá, Maringá, Paraná, 87020-900, Brazil.
3
Biotechnology Applied to the Agriculture, Paranaense University, Umuarama, Paraná, 87.502-210, Brazil.
4
Department of Chemistry, State University of Maringá, Maringá, Paraná, 87020-900, Brazil.
5
Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, Paraná, 87020-900, Brazil.
6
Health Promotion, Cesumar University, Maringá, Paraná, 87.050-900, Brazil.
7
Clean Technologies and Cesumar Institute of Science, Technology and Innovation - ICETI, Cesumar University, Maringá, Paraná, 87.050-900, Brazil.
8
Biotechnology Applied to the Agriculture, Paranaense University, Umuarama, Paraná, 87.502-210, Brazil. cristianigazim@unipar.br.
9
Laboratory of Chemistry of Natural Products, Paranaense University, Mascarenhas de Moraes Square, 4282, Umuarama, PR, Brazil. cristianigazim@unipar.br.

Abstract

Tetradenia riparia (Lamiaceae) is native to Central Africa popularly known as myrrh, used in folk medicine to treat various diseases like malaria, gastroenteritis, and tropical skin disease. This research was to evaluate the antioxidant and antibacterial activities of the crude extract (CE) and fractions (FR) of the T. riparia by classical chromatography. The CE of T. riparia leaves was submitted to column chromatographic fractionation to obtain four fractions of the interest, which were identified by nuclear magnetic resonance and gas chromatograph coupled to mass spectrum: FR-I (abieta-7,9(11)-dien-13-β-ol), FR-II (Ibozol), FR-III (8 (14), 15-sandaracopimaradiene-2α, 18-diol and 8 (14), 15-sandaracopimaradiene-7α, 18-diol), and FR-IV (Astragalin, Boronolide and Luteolin). Total phenol content of CE and FR were measured, and antioxidant action by methods of DPPH (2,2-diphenyl-1-picrylhydrazyl), β-carotene/linoleic acid system, and ferric reducing/antioxidant power (FRAP) and the antibacterial activity was evaluated by the broth microdilution method with the determination of the minimum inhibitory concentration (MIC). The FR-IV presented antioxidant potential with 181.67 μg gallic acid/mg, IC50 of 0.61 μg/mL by DPPH method, 55.61% oxidation protection by β-carotene/linoleic acid system and 4.59 µM ferrous sulfate/mg of sample by FRAP, and the FR-I showed higher antibacterial potential on the strain Staphylococcus aureus with MIC 0.98 μg/mL, Enterococcus faecalis and Bacillus cereus with MIC 31.2 μg/mL. Thus, the fractionation of CE was extremely important to detect fractions with potential activities, and investigations are necessary regarding the mechanism of action and action in vivo.

PMID:
28840299
DOI:
10.1007/s00284-017-1340-9
[Indexed for MEDLINE]

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