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Sci Rep. 2017 Aug 24;7(1):9325. doi: 10.1038/s41598-017-10067-w.

SmartScope2: Simultaneous Imaging and Reconstruction of Neuronal Morphology.

Author information

1
Allen Institute for Brain Science, Seattle, WA, 98109, USA.
2
Department of Neurosurgery, Swedish Neuroscience Institute, Seattle, WA, 98122, USA.
3
Allen Institute for Brain Science, Seattle, WA, 98109, USA. hanchuanp@alleninstitute.org.

Abstract

Quantitative analysis of neuronal morphology is critical in cell type classification and for deciphering how structure gives rise to function in the brain. Most current approaches to imaging and tracing neuronal 3D morphology are data intensive. We introduce SmartScope2, the first open source, automated neuron reconstruction machine integrating online image analysis with automated multiphoton imaging. SmartScope2 takes advantage of a neuron's sparse morphology to improve imaging speed and reduce image data stored, transferred and analyzed. We show that SmartScope2 is able to produce the complex 3D morphology of human and mouse cortical neurons with six-fold reduction in image data requirements and three times the imaging speed compared to conventional methods.

PMID:
28839271
PMCID:
PMC5571186
DOI:
10.1038/s41598-017-10067-w
[Indexed for MEDLINE]
Free PMC Article

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