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Genes Dev. 2017 Jul 15;31(14):1494-1508. doi: 10.1101/gad.300855.117. Epub 2017 Aug 24.

Histone locus regulation by the Drosophila dosage compensation adaptor protein CLAMP.

Author information

1
Department of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912, USA.
2
Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
3
Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
4
Integrative Program for Biological and Genome Sciences, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
5
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
6
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
7
Department of Biology, University of North Carolina at Chapel Hill, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
8
Department of Genetics, University of North Carolina, Chapel Hill, North Carolina 27599, USA.

Abstract

The conserved histone locus body (HLB) assembles prior to zygotic gene activation early during development and concentrates factors into a nuclear domain of coordinated histone gene regulation. Although HLBs form specifically at replication-dependent histone loci, the cis and trans factors that target HLB components to histone genes remained unknown. Here we report that conserved GA repeat cis elements within the bidirectional histone3-histone4 promoter direct HLB formation in Drosophila In addition, the CLAMP (chromatin-linked adaptor for male-specific lethal [MSL] proteins) zinc finger protein binds these GA repeat motifs, increases chromatin accessibility, enhances histone gene transcription, and promotes HLB formation. We demonstrated previously that CLAMP also promotes the formation of another domain of coordinated gene regulation: the dosage-compensated male X chromosome. Therefore, CLAMP binding to GA repeat motifs promotes the formation of two distinct domains of coordinated gene activation located at different places in the genome.

KEYWORDS:

CLAMP; GA repeats; GAGA factor; dosage compensation; histone locus body; nuclear bodies

PMID:
28838946
PMCID:
PMC5588930
DOI:
10.1101/gad.300855.117
[Indexed for MEDLINE]
Free PMC Article

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