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J Virol Methods. 2017 Nov;249:85-93. doi: 10.1016/j.jviromet.2017.08.012. Epub 2017 Aug 31.

Development of a recombinant yellow fever vector expressing a HIV clade C founder envelope gp120.

Author information

1
Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, NC 27710, United States. Electronic address: jaesung.yu@duke.edu.
2
Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, NC 27710, United States.
3
Infectious Disease Research Institute, Seattle, WA 98102, United States.
4
Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY, 10065, United States.
5
Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, NC 27710, United States. Electronic address: hayne002@mc.duke.edu.

Abstract

Development of a HIV-1 vaccine is a major global priority. The yellow fever virus (YFV) attenuated vaccine 17D is among the most effective of currently used vaccines. However, the stability of the YFV17D vector when carrying non-flavivirus genes has been problematic. We have constructed and expressed HIV-1 Env in YFV17D with either single transmembrane (STM) or double transmembrane (DTM) YFV E protein domains for the development of anti-HIV antibodies. Here we describe modifications of the YFV17D vector such that HIV-1 Env gp120 is expressed in up to 5 passages in Vero cells. Immunization with recombinant YFV17D vector prime followed by HIV-1 CH505 TF gp120 protein boosts were able to induce neutralizing antibodies for a HIV-1 tier 1 isolate in mice. This modified YFV vector may be a starting point for constructing HIV-1 vaccine candidate priming vectors.

KEYWORDS:

Antibodies; HIV; HIV envelope; Neutralization; Transmitter/founder; YFV17D

PMID:
28837840
PMCID:
PMC5623118
DOI:
10.1016/j.jviromet.2017.08.012
[Indexed for MEDLINE]
Free PMC Article

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