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Bioprocess Biosyst Eng. 2017 Dec;40(12):1763-1773. doi: 10.1007/s00449-017-1831-x. Epub 2017 Aug 23.

Scale-up cultivation enhanced arachidonic acid accumulation by red microalgae Porphyridium purpureum.

Author information

1
College of Energy, Xiamen University, Xiamen, 361102, People's Republic of China.
2
College of Energy, Xiamen University, Xiamen, 361102, People's Republic of China. xianhai.zeng@xmu.edu.cn.
3
Xiamen Key Laboratory of High-valued Conversion Technology of Agricultural Biomass, Xiamen University, Xiamen, 361102, People's Republic of China. xianhai.zeng@xmu.edu.cn.
4
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, People's Republic of China.
5
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, People's Republic of China.
6
Xiamen Key Laboratory of High-valued Conversion Technology of Agricultural Biomass, Xiamen University, Xiamen, 361102, People's Republic of China.
7
College of Energy, Xiamen University, Xiamen, 361102, People's Republic of China. lulin@xmu.edu.cn.
8
Xiamen Key Laboratory of High-valued Conversion Technology of Agricultural Biomass, Xiamen University, Xiamen, 361102, People's Republic of China. lulin@xmu.edu.cn.

Abstract

The present study attempts to cultivate Porphyridium purpureum under different scale-up conditions for further development and commercialization of microalgae-derived PUFAs such as ARA and EPA. Different temperatures (25, 30, and 35 °C) and light intensities (70, 165, and 280 μmol/m2s) were applied to the 50 L pilot-scale cultivation of P. purpureum in ASW. The cultivation under the light intensity of 280 μmol/m2s at 35 °C obtained biomass concentration up to 9.52 g/L, total fatty acid content to 56.82 mg/g, and ARA content to 22.29 mg/g. While the maximum EPA content of 7.00 mg/g was achieved under the light intensity of 280 μmol/m2s at 25 °C and the highest ratio of UFAs to TFAs of 74.66% was also obtained in this trial. Both biomass concentration and TFAs content were improved by increasing light intensity and temperature. Moreover, the ratio of ARA to EPA was enhanced by increasing cultivation temperature under the light intensity of 280 μmol/m2s. In contrast with flask culture, the conversion of linoleic acid (C18:2) to ARA was enhanced in scale-up culture, leading to more ARA content. Phosphate limitation enhanced the synthesis of lipid and LPUFAs. Moreover, the biomass concentration and biosynthesis of palmitic acid were preferred by sufficient C (NaHCO3).

KEYWORDS:

Arachidonic acid; Enhance; Phosphate limitation; Porphyridium purpureum; Scale-up cultivation

PMID:
28836004
DOI:
10.1007/s00449-017-1831-x
[Indexed for MEDLINE]

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