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Angew Chem Int Ed Engl. 2017 Sep 18;56(39):11916-11920. doi: 10.1002/anie.201703807. Epub 2017 Aug 21.

Aptamer/AuNP Biosensor for Colorimetric Profiling of Exosomal Proteins.

Jiang Y1,2, Shi M1,2, Liu Y1,2, Wan S2, Cui C2, Zhang L1,2, Tan W1,2.

Author information

1
Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering and College of Life Sciences, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan, 410082, China.
2
Department of Chemistry, Department of Physiology and Functional Genomics, Center for Research at Bio/nano Interface, UF Health Cancer Center, UF Genetics Institute, University of Florida, Gainesville, FL, 32611, USA.

Abstract

Exosomes constitute an emerging biomarker for cancer diagnosis because they carry multiple proteins that reflect the origins of parent cells. Assessing exosome surface proteins provides a powerful means of identifying a combination of biomarkers for cancer diagnosis. We report a sensor platform that profiles exosome surface proteins in minutes by the naked eye. The sensor consists of a gold nanoparticle (AuNP) complexed with a panel of aptamers. The complexation of aptamers with AuNPs protects the nanoparticles from aggregating in a high-salt solution. In the presence of exosomes, the non-specific and weaker binding between aptamers and the AuNP is broken, and the specific and stronger binding between exosome surface protein and the aptamer displaces aptamers from the AuNP surface and results in AuNP aggregation. This aggregation results in a color change and generates patterns for the identification of multiple proteins on the exosome surface.

KEYWORDS:

aptamers; exosome biomarkers; gold nanoparticles; molecular recognition; protein profiling

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