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Pharm Biol. 2017 Dec;55(1):2095-2101. doi: 10.1080/13880209.2017.1357737.

Anti-inflammatory effect of pomegranate flower in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages.

Xu J1,2, Zhao Y1,3, Aisa HA1,3.

Author information

1
a Key Laboratory of Chemistry of Plant Resources in Arid Regions , Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Urumqi , China.
2
b University of Chinese Academy of Sciences , Beijing , China.
3
c State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization , Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Urumqi , China.

Abstract

CONTEXT:

Punica granatum L (Punicaceae) flower is an important diabetes treatment in oriental herbal medicine.

OBJECTIVE:

This study investigates the inflammation effects of pomegranate flower (PFE) ethanol extract in LPS-induced RAW264.7 cells.

MATERIALS AND METHODS:

PFE (10, 25, 50, 100 μg/mL) was applied to 1 μg/mL LPS-induced RAW 264.7 macrophages in vitro. Levels of nitric oxide (NO), prostaglandin E2 (PGE2) and pro-inflammatory cytokines interleukin (IL)-1β (IL-1β), interleukin (IL)-6 (IL-6) and tumor necrosis factor (TNF-α) in the supernatant fraction were determined using enzyme-linked immunosorbent assay (ELISA). Expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), phosphorylation of mitogen-activated protein kinase (MAPK) subgroups extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and P38, as well as nuclear factor-κB (NF-κB) activation in extracts were detected via Western blot.

RESULTS:

10-100 μg/mL PFE decreased the production of NO (IC50 value = 31.8 μg/mL), PGE2 (IC50 value = 54.5 μg/mL), IL-6 (IC50 value = 48.7 μg/mL), IL-1β (IC50 value = 71.3 μg/mL) and TNF-α (IC50 value = 62.5 μg/mL) in LPS-stimulated RAW 264.7 cells significantly. A mechanism-based study showed that phosphorylation of ERK1/2, p38, JNK and translocation of the NF-B p65 subunit into nuclei were inhibited by the PFE treatment.

DISCUSSION AND CONCLUSION:

These results show that PFE produced potential anti-inflammatory effect through modulating the synthesis of several mediators and cytokines involved in the inflammatory process.

KEYWORDS:

MAPK; NF-κB; Punica granatum; anti-inflammation

PMID:
28832232
PMCID:
PMC6130474
DOI:
10.1080/13880209.2017.1357737
[Indexed for MEDLINE]
Free PMC Article

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