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Genomics. 2018 Jan;110(1):59-66. doi: 10.1016/j.ygeno.2017.08.006. Epub 2017 Aug 19.

Influence of epistatic segregation distortion loci on genetic marker linkages in Japanese flounder.

Author information

1
Key Laboratory of Aquatic Genomics, Ministry of Agriculture, Research Centre for Aquatic Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, China.
2
Department of Biological Science and Technology, Heilongjiang Vocational College for Nationalities, Harbin 150066, China.
3
Division of Comprehensive Aquaculture, Shandong Weihai Institute of Ocean and Aquaculture, Weihai 264200, China.
4
Key Laboratory of Aquatic Genomics, Ministry of Agriculture, Research Centre for Aquatic Biotechnology, Chinese Academy of Fishery Sciences, Beijing 100141, China. Electronic address: runqingyang@cafs.ac.cn.

Abstract

For genetic linkage analysis of Japanese flounder, 160 doubled haploids (DH) were artificially produced using mitotic gynogenesis and were genotyped for 458 simple sequence repeat (SSR) markers, 101 of which show distortional segregation. The genetic linkage map was constructed by modifying recombination fractions between the distorted markers. Between the corrected and uncorrected genetic maps, there were considerable differences in genetic distance, but not in relative locations among markers. Using a liability model, a segregation distortion locus (SDL), with an additive genetic effect of 1.772, was mapped between markers BDHYP387 and Poli56TUF of chromosome 24 in the corrected genetic map. Additionally, six pairs of epistatic SDLs were identified on chromosomes 1, 5, 8, 9, 23, and 24. Changes in genetic distances between markers did not occur on chromosome regions with main effect SDLs. However, most chromosome regions where genetic distances changed covered the detected epistatic SDLs. This study concluded that epistatic SDLs decrease linkages between markers and lengthen genetic distances in Japanese flounder. This finding has been partially validated in other DH populations derived from three female Japanese flounders.

KEYWORDS:

Distorted segregation; Epistasis; Genetic linkage map; Japanese flounder; SDL

PMID:
28830780
DOI:
10.1016/j.ygeno.2017.08.006
[Indexed for MEDLINE]

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