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Int J Biol Macromol. 2018 Jan;106:947-954. doi: 10.1016/j.ijbiomac.2017.08.096. Epub 2017 Aug 19.

Polysaccharide from Ephedra sinica Stapf inhibits inflammation expression by regulating Factor-β1/Smad2 signaling.

Author information

1
Heilongjiang University of Chinese Medicine, Key Laboratory of Basic and Applied Research in North Medicine, Ministry of Education, Heilongjiang Key Laboratory of Drug Efficacy Study Material of Traditional Chinese Medicine and Natural Product, Harbin, 150040, China. Electronic address: lss988723@163.com.
2
Heilongjiang University of Chinese Medicine, Key Laboratory of Basic and Applied Research in North Medicine, Ministry of Education, Heilongjiang Key Laboratory of Drug Efficacy Study Material of Traditional Chinese Medicine and Natural Product, Harbin, 150040, China. Electronic address: 1446569115@qq.com.
3
Heilongjiang University of Chinese Medicine, Key Laboratory of Basic and Applied Research in North Medicine, Ministry of Education, Heilongjiang Key Laboratory of Drug Efficacy Study Material of Traditional Chinese Medicine and Natural Product, Harbin, 150040, China. Electronic address: 5363646@qq.com.
4
Heilongjiang University of Chinese Medicine, Key Laboratory of Basic and Applied Research in North Medicine, Ministry of Education, Heilongjiang Key Laboratory of Drug Efficacy Study Material of Traditional Chinese Medicine and Natural Product, Harbin, 150040, China. Electronic address: 1728970605@qq.com.
5
Heilongjiang University of Chinese Medicine, Key Laboratory of Basic and Applied Research in North Medicine, Ministry of Education, Heilongjiang Key Laboratory of Drug Efficacy Study Material of Traditional Chinese Medicine and Natural Product, Harbin, 150040, China. Electronic address: hxkuang588@163.com.
6
Guangdong Pharmaceutical University, 510224, China. Electronic address: qhwang588@163.com.

Abstract

Mahuang, a famous traditional Chinese medicine, is widely used for the treatment of common cold and inflammatory diseases in China. We investigated the protective effects of polysaccharide from Ephedra sinica Stapf (ESP-B4) against airway and pulmonary inflammation. Animal model was made in SD rats via intratracheal instillation of Lipopolysaccharides (LPS) on day1 and 14 and inhalation of Cigarette Smoke (CS) for 4 weeks. ESP-B4 was administered by oral gavage for 2 weeks. We studied the number of inflammatory cells in serum, production of TNF-α, IL-6, IL-8 and MMP-9 in lung homogenization buffer, and the expression of TGF-β1, P-Smad2 and P-Smad3 in lung tissue. In vitro, model was made in A549 cells with LPS and cigarette smoke extract. We investigated the levels of TNF-α, IL-6 and IL-8, and the expression of TGF-β1, P-Smad2, P-Smad3 and Smad7. Our results revealed that ESP-B4 lowered the recruitment of inflammatory cells; and decreased the production of TNF-α, IL-6, IL-8 and MMP-9, significantly reduced the expression of TGF-β1, P-Smad2 and P-Smad3, increased the expression of Smad7. These results indicated that ESP-B4 reduced airway and pulmonary inflammation by regulating inflammatory cytokines and the TGF-β1/Smad2 pathway, thus providing a potential treatment for chronic obstructive pulmonary diseases (COPD).

PMID:
28830773
DOI:
10.1016/j.ijbiomac.2017.08.096
[Indexed for MEDLINE]

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