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PLoS One. 2017 Aug 22;12(8):e0183487. doi: 10.1371/journal.pone.0183487. eCollection 2017.

Nanofibrillar cellulose-alginate hydrogel coated surgical sutures as cell-carrier systems.

Author information

1
Division of Pharmaceutical Biosciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.
2
Department of Engineering Design and Production, School of Engineering, Aalto University, Espoo, Finland.
3
School of Pharmacy, University of Eastern Finland, Kuopio, Finland.
4
Made Consulting, Turku, Finland.
5
Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland.
6
Department of Otorhinolaryngology-Head and Neck Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.

Abstract

Hydrogel nanomaterials, especially those that are of non-human and non-animal origins, have great potential in biomedical and pharmaceutical sciences due to their versatility and inherent soft-tissue like properties. With the ability to simulate native tissue function, hydrogels are potentially well suited for cellular therapy applications. In this study, we have fabricated nanofibrillar cellulose-alginate (NFCA) suture coatings as biomedical devices to help overcome some of the limitations related to cellular therapy, such as low cell survivability and distribution out of target tissue. The addition of sodium alginate 8% (w/v) increased the NFCA hydrogel viscosity, storage and loss moduli by slightly under one order of magnitude, thus contributing significantly to coating strength. Confocal microscopy showed nearly 100% cell viability throughout the 2-week incubation period within and on the surface of the coating. Additionally, typical morphologies in the dual cell culture of spheroid forming HepG2 and monolayer type SK-HEP-1 were observed. Twelve out of 14 NFCA coated surgical sutures remained intact during the suturing operation with various mice and rat tissue; however, partial peeling off was observed in 2 of the coated sutures. We conclude that NFCA suture coatings could perform as cell-carrier systems for cellular based therapy and post-surgical treatment.

PMID:
28829830
PMCID:
PMC5567492
DOI:
10.1371/journal.pone.0183487
[Indexed for MEDLINE]
Free PMC Article

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