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PLoS One. 2017 Aug 22;12(8):e0183292. doi: 10.1371/journal.pone.0183292. eCollection 2017.

Effect of endurance exercise on microRNAs in myositis skeletal muscle-A randomized controlled study.

Author information

1
Research Center for Genetic Medicine, Children's National Health System, Washington, D.C., United States of America.
2
Department of Integrative Systems Biology, Institute for Biomedical Sciences, The George Washington University, Washington, D.C., United States of America.
3
Rheumatology Unit, Department of Medicine, Solna, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.
4
Departement of Pharmaceuticals Sciences, School of Pharmacy and Pharmaceuticals Sciences, Binghamton University, Binghamton, New York, United States of America.

Abstract

OBJECTIVE:

To identify changes in skeletal muscle microRNA expression after endurance exercise and associate the identified microRNAs with mRNA and protein expression to disease-specific pathways in polymyositis (PM) and dermatomyositis (DM) patients.

METHODS:

Following a parallel clinical trial design, patients with probable PM or DM, exercising less than once a week, and on stable medication for at least one month were randomized into two groups at Karolinska University Hospital: a 12-week endurance exercise group (n = 12) or a non-exercised control group (n = 11). Using an Affymetrix microarray, microRNA expression was determined in paired muscle biopsies taken before and after the exercise intervention from 3 patients in each group. Ingenuity pathway analysis with a microRNA target filter was used to identify microRNA transcript targets. These targets were investigated at the mRNA (microarray) and protein (mass spectrometry) levels in patients.

RESULTS:

Endurance exercise altered 39 microRNAs. The microRNAs with increased expression were predicted to target transcripts involved in inflammatory processes, metabolism, and muscle atrophy. Further, these target transcripts had an associated decrease in mRNA expression in exercised patients. In particular, a decrease in the NF-κB regulator IKBKB was associated with an increase in its target microRNA (miR-196b). At the protein level, there was an increase in mitochondrial proteins (AK3, HIBADH), which were associated with a decrease in microRNAs that were predicted to regulate their expression.

CONCLUSION:

Improvement in disease phenotype after exercise is associated with increasing microRNAs that target and downregulate immune processes at the transcript level, as well as decreasing microRNAs that target and upregulate mitochondrial content at the protein level. Therefore, microRNAs may improve disease by decreasing immune responses and increasing mitochondrial biogenesis.

TRIAL REGISTRATION:

ClinicalTrials.gov NCT01184625.

PMID:
28829792
PMCID:
PMC5568726
DOI:
10.1371/journal.pone.0183292
[Indexed for MEDLINE]
Free PMC Article

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