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Appl Spectrosc. 2017 Dec;71(12):2595-2607. doi: 10.1177/0003702817715042. Epub 2017 Aug 22.

Label-Free Raman Hyperspectral Imaging of Single Cells Cultured on Polymer Substrates.

Author information

1
1 School of Physics and Astronomy, University of Nottingham, Nottingham, UK.
2
2 School of Pharmacy, University of Nottingham, Nottingham, UK.

Abstract

While Raman hyperspectral imaging has been widely used for label-free mapping of biomolecules in cells, these measurements require the cells to be cultured on weakly Raman scattering substrates. However, many applications in biological sciences and engineering require the cells to be cultured on polymer substrates that often generate large Raman scattering signals. Here, we discuss the theoretical limits of the signal-to-noise ratio in the Raman spectra of cells in the presence of polymer signals and how optical aberrations may affect these measurements. We show that Raman spectra of cells cultured on polymer substrates can be obtained using automatic subtraction of the polymer signals and demonstrate the capabilities of these methods in two important applications: tissue engineering and in vitro toxicology screening of drugs. Apart from their scientific and technological importance, these applications are examples of the two most common measurement configurations: (1) cells cultured on an optically thick polymer substrate measured using an immersion/dipping objective; and (2) cells cultured on a transparent polymer substrate and measured using an inverted optical microscope. In these examples, we show that Raman hyperspectral data sets with sufficient quality can be successfully acquired to map the distribution of common biomolecules in cells, such as nucleic acids, proteins, and lipids, as well as detecting the early stages of apoptosis. We also discuss strategies for further improvements that could expand the application of Raman hyperspectral imaging on polymer substrates even further in biomedical sciences and engineering.

KEYWORDS:

Raman spectroscopy; background correction; cells; hyperspectral imaging; polymer substrates

PMID:
28828895
PMCID:
PMC5703035
DOI:
10.1177/0003702817715042
[Indexed for MEDLINE]
Free PMC Article

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