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J Struct Biol. 2017 Sep;199(3):225-236. doi: 10.1016/j.jsb.2017.07.011. Epub 2017 Aug 4.

Best practices for managing large CryoEM facilities.

Author information

1
NeCEN, Leiden University, Netherlands.
2
Diamond Light Source, United Kingdom.
3
Harvard Medical School, United States.
4
California Institute of Technology, United States.
5
New York Structural Biology Center, United States.
6
The Rockefeller University, United States.
7
EMBL Heidelberg, Germany.
8
Oregon Health & Science University, United States.
9
ISMB/Birkbeck, United Kingdom.
10
McGill University, Canada.
11
FEI Company/Thermo Fisher Scientific, United States.
12
Merck & Co, Inc., United States.
13
Florida State University, United States.
14
Northwestern University, United States.
15
Emory University School of Medicine, United States.
16
Yale University, United States.
17
HHMI Janelia Research Campus, United States.
18
University of California, Los Angeles, United States.
19
New York Structural Biology Center, United States. Electronic address: cpotter@nysbc.org.

Abstract

This paper provides an overview of the discussion and presentations from the Workshop on the Management of Large CryoEM Facilities held at the New York Structural Biology Center, New York, NY on February 6-7, 2017. A major objective of the workshop was to discuss best practices for managing cryoEM facilities. The discussions were largely focused on supporting single-particle methods for cryoEM and topics included: user access, assessing projects, workflow, sample handling, microscopy, data management and processing, and user training.

KEYWORDS:

Automation; Cryotem; Data management; Facility management; Single-particle; User training

PMID:
28827185
PMCID:
PMC5605453
DOI:
10.1016/j.jsb.2017.07.011
[Indexed for MEDLINE]
Free PMC Article

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