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Elife. 2017 Aug 15;6. pii: e27713. doi: 10.7554/eLife.27713.

Environmental cystine drives glutamine anaplerosis and sensitizes cancer cells to glutaminase inhibition.

Author information

1
Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, United States.
2
Whitehead Institute for Biomedical Research, Cambridge, United States.
3
Dana-Farber Cancer Institute, Boston, United States.

Abstract

Many mammalian cancer cell lines depend on glutamine as a major tri-carboxylic acid (TCA) cycle anaplerotic substrate to support proliferation. However, some cell lines that depend on glutamine anaplerosis in culture rely less on glutamine catabolism to proliferate in vivo. We sought to understand the environmental differences that cause differential dependence on glutamine for anaplerosis. We find that cells cultured in adult bovine serum, which better reflects nutrients available to cells in vivo, exhibit decreased glutamine catabolism and reduced reliance on glutamine anaplerosis compared to cells cultured in standard tissue culture conditions. We find that levels of a single nutrient, cystine, accounts for the differential dependence on glutamine in these different environmental contexts. Further, we show that cystine levels dictate glutamine dependence via the cystine/glutamate antiporter xCT/SLC7A11. Thus, xCT/SLC7A11 expression, in conjunction with environmental cystine, is necessary and sufficient to increase glutamine catabolism, defining important determinants of glutamine anaplerosis and glutaminase dependence in cancer.

KEYWORDS:

SLC7A11; cancer; cancer biology; cystine; environment; glutaminase; human; metabolism; mouse

PMID:
28826492
PMCID:
PMC5589418
DOI:
10.7554/eLife.27713
[Indexed for MEDLINE]
Free PMC Article

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