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Elife. 2017 Aug 9;6. pii: e26343. doi: 10.7554/eLife.26343.

Eyeless uncouples mushroom body neuroblast proliferation from dietary amino acids in Drosophila.

Author information

1
Department of Biology, University of Virginia, Charlottesville, United States.

Abstract

Cell proliferation is coupled with nutrient availability. If nutrients become limited, proliferation ceases, because growth factor and/or PI3-kinase activity levels become attenuated. Here, we report an exception to this generality within a subpopulation of Drosophila neural stem cells (neuroblasts). We find that most neuroblasts enter and exit cell cycle in a nutrient-dependent manner that is reversible and regulated by PI3-kinase. However, a small subset, the mushroom body neuroblasts, which generate neurons important for memory and learning, divide independent of dietary nutrient conditions and PI3-kinase activity. This nutrient-independent proliferation is regulated by Eyeless, a Pax-6 orthologue, expressed in mushroom body neuroblasts. When Eyeless is knocked down, mushroom body neuroblasts exit cell cycle when nutrients are withdrawn. Conversely, when Eyeless is ectopically expressed, some non-mushroom body neuroblasts divide independent of dietary nutrient conditions. Therefore, Eyeless uncouples MB neuroblast proliferation from nutrient availability, allowing preferential neurogenesis in brain subregions during nutrient poor conditions.

KEYWORDS:

D. melanogaster; PI3-kinase; Pax-6; developmental biology; diet; eyeless; neural stem cell; neuroblast; stem cells

PMID:
28826476
PMCID:
PMC5576483
DOI:
10.7554/eLife.26343
[Indexed for MEDLINE]
Free PMC Article

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