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Sci Total Environ. 2018 Jan 1;610-611:769-775. doi: 10.1016/j.scitotenv.2017.08.052. Epub 2017 Aug 18.

Acute and chronic toxic effects of fluoxastrobin on zebrafish (Danio rerio).

Author information

1
College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China.
2
College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China. Electronic address: jwang@sdau.edu.cn.
3
College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China. Electronic address: lushzhu@sdau.edu.cn.
4
College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China. Electronic address: wjh@sdau.edu.cn.

Abstract

Fluoxastrobin is a new strobilurin fungicide, similar to azoxystrobin and pyraclostrobin. Before the wide application of fluoxastrobin, the present study was performed to assay the acute and chronic toxicity of fluoxastrobin on zebrafish (Danio rerio). The 96-hour median lethal concentration (96h LC50) after initiation of zebrafish exposure to fluoxastrobin was 0.51mg/L with a 95% confidence interval of 0.45 to 0.57mg/L, indicating that fluoxastrobin was highly toxic to zebrafish. As endpoints, we assayed the levels of reactive oxygen species (ROS), malondialdehyde (MDA), the activities of superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and the degree of DNA damage at three different doses, 0.001, 0.01, and 0.1mg/L on days 7, 14, 21, and 28. The antioxidant enzymes partially ameliorated the ROS induced by fluoxastrobin t and were in turn inhibited by excess ROS, especially at 0.1mg/L. Lipid peroxidation and DNA damage were stimulated by ROS. The fluoxastrobin contents of the tested solutions were also determined; at the fluoxastrobin doses of 0.001, 0.01, and 0.1mg/L, the contents on day 28 were 3.9, 5.0, and 0.64% greater than those on day 0. Thus, fluoxastrobin was relatively stable in an aquatic environment. In addition, the present study provided more information regarding the toxic effects of fluoxastrobin and the scientific methods for selection and evaluation of fungicides in the future.

KEYWORDS:

96h LC(50); Antioxidant enzyme; DNA damage; Genetic toxicity; High-performance liquid chromatography; Reactive oxygen species

PMID:
28826114
DOI:
10.1016/j.scitotenv.2017.08.052
[Indexed for MEDLINE]

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