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Nat Chem Biol. 2017 Oct;13(10):1109-1114. doi: 10.1038/nchembio.2459. Epub 2017 Aug 21.

Metals induce transient folding and activation of the twister ribozyme.

Author information

1
T.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland, USA.
2
Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
3
Howard Hughes Medical Institute, Baltimore, Maryland, USA.

Abstract

Twister is a small ribozyme present in almost all kingdoms of life that rapidly self-cleaves in variety of divalent metal ions. We used activity assays, bulk FRET and single-molecule FRET (smFRET) to understand how different metal ions promote folding and self-cleavage of the Oryza sativa twister ribozyme. Although most ribozymes require additional Mg2+ for catalysis, twister inverts this expectation, requiring 20-30 times less Mg2+ to self-cleave than to fold. Transition metals such as Co2+, Ni2+ and Zn2+ activate twister more efficiently than Mg2+ ions. Although twister is fully active in ≤ 0.5 mM MgCl2, smFRET experiments showed that the ribozyme visits the folded state infrequently under these conditions. Comparison of folding and self-cleavage rates indicates that most folding events lead to catalysis, which correlates with metal bond strength. Thus, the robust activity of twister reports on transient metal ion binding under physiological conditions.

PMID:
28825710
PMCID:
PMC5605428
DOI:
10.1038/nchembio.2459
[Indexed for MEDLINE]
Free PMC Article

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