Format

Send to

Choose Destination
J Fluoresc. 2017 Nov;27(6):2231-2238. doi: 10.1007/s10895-017-2164-5. Epub 2017 Aug 19.

Fluorescent Labeling of Protein Using Blue-Emitting 8-Amino-BODIPY Derivatives.

Kim D1,2,3, Ma D4, Kim M5, Jung Y5, Kim NH5, Lee C6, Cho SW4, Park S6, Huh Y7,5, Jung J7,5, Ahn KH4.

Author information

1
Department of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, Seoul, 02447, South Korea. dkim@khu.ac.kr.
2
Center for Converging Humanities, Kyung Hee University, Seoul, 02447, South Korea. dkim@khu.ac.kr.
3
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, 02447, South Korea. dkim@khu.ac.kr.
4
Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, Gyungbuk, 37673, South Korea.
5
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, 02447, South Korea.
6
Department of Chemistry, Korea University, Seoul, 02841, South Korea.
7
Department of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, Seoul, 02447, South Korea.

Abstract

8-Amino-BODIPY (boron-dipyrromethane) dyes show bright blue fluorescence. Disclosed here are synthesis and characterization of the photophysical properties of a series of functionalized 8-Amino-BODIPY (BP1-4) for protein labeling. The compact structure and solvent-insensitive absorption property of the dye are desirable features for protein labeling. For the model protein, bovine serum albumin (BSA), the labeling proceeds under mild condition via amide bond formation or thiol-ene conjugation with maintaining the bright blue fluorescence. The chromatography and mass spectroscopy analysis clearly support the labeling of the BODIPY dye on the BSA. The protein labeling with blue-emitting BODIPY would be applicable for studying protein dynamics and fluorescence resonance energy transfer (FRET) with intrinsic biomolecules.

KEYWORDS:

Bioconjugation; Blue-emitting BODIPY; Fluorescent labeling; Protein labeling

PMID:
28823107
DOI:
10.1007/s10895-017-2164-5
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center