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Pathol Res Pract. 2017 Sep;213(9):1166-1172. doi: 10.1016/j.prp.2017.06.017. Epub 2017 Jun 28.

Decreased expression of microRNA-122 is associated with an unfavorable prognosis in childhood acute myeloid leukemia and function analysis indicates a therapeutic potential.

Author information

1
Department of Pediatrics, Huai'an Hospital Affiliated to Xuzhou Medical College and Huai'an Second People's Hospital, 62 Huaihai Road South, Huai'an 223002, China.
2
Department of Pediatrics, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, China.
3
Department of Pediatrics, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, China. Electronic address: yljhayy@163.com.

Abstract

MicroRNA (miR)-122 functions as a tumor suppressor in various human cancers. However, its involvement in childhood acute myeloid leukemia (AML) remains unknown. In this study, quantitative real-time PCR assay demonstrated that miR-122 expression in bone marrow specimens from AML children were significantly lower than that in non-malignant controls (P<0.001). Statistically, AML children with low miR-122 expression more frequently had large white blood cell count (P=0.022), French-American-British classification subtype M7 (P<0.001), unfavorable cytogenetics (P=0.002) and day 7 response to the treatment (P=0.036), short relapse-free (P=0.001) and overall (P=0.008) survivals than those with high expression. Multivariate analysis also determined that miR-122 expression was an independent prognostic factor for both relapse-free and overall survivals. Functionally, the enforced expression of miR-122 in AML cell lines efficiently suppressed cell proliferation and reduced the ratio of S-phase cells in vitro (all P<0.05). In conclusion, the abnormal expression of miR-122 may be a marker of the aggressive progression in childhood AML. Importantly, its downregulation may serve as a prognostic factor to predict poor outcome. Our study also reveal that miR-122 may function as a tumor suppressor in childhood AML, highlighting a new therapeutic strategy for this malignancy.

KEYWORDS:

Cell proliferation; Childhood acute myeloid leukemia; Prognosis; Tumor suppressor; microRNA-122

PMID:
28822593
DOI:
10.1016/j.prp.2017.06.017
[Indexed for MEDLINE]

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