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Zhongguo Zhong Yao Za Zhi. 2017 Jun;42(12):2305-2310. doi: 10.19540/j.cnki.cjcmm.20170426.004.

[Analyzing crude/processed root of Polygonum multiflorum from different habitats by UPLC fingerprint and mode identification methods].

[Article in Chinese]
Xiao R1,2, Lin Y1,2, Lei SM1,2, Zhang Y1,2, Huang J1,2, Xia BH1,2, Li C3, Liao DF1,2, Wu P1,2, Lin LM1,2.

Author information

1
College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
2
Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Changsha 410208, China.
3
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.

Abstract

To establish a content determination method for 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside (TSG) of the crude/processed root of Polygonum multiflorum from different habitats in China and set up the fingerprint by using UPLC. Various samples were pretreated by macro-porous resin. Then UPLC analysis was performed on Waters ACQUITY UPLC@BEH C18 chromatographic column (2.1 mm×50 mm, 1.7 μm) at (25±5) ℃. A binary gradient elution system was composed of acetonitrile (phase A) and 0.5% acetic acid solution (phase B). Detection was performed at the wavelength of 254 nm, and the mobile flow rate was set at 0.3 mL•min⁻¹. Results showed that the yield of extraction of the 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside from root of P. multiflorum was all over 25.0% after macro-porous resin separation; an exclusive UPLC fingerprint method of the crude/processed root of P. multiflorum from different habitats was successfully set up and 17 chromatographic peaks were calibrated. Cluster analysis can not entirely distinguish the crude one from the processed one, while principal component analysis absolutely can. 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside is the composition that has largest differences in variable importance in projection (VIP) between crude and processed root of P. multiflorum. The separating method can gain high-purity 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside, and the determination method is simple, sensitive, reliable and can be used in fast identifying the crude/processed root of P. multiflorum or as a method for overall quality control of root of P. multiflorum.

KEYWORDS:

2,3,5,4′-tetrahydroxy stilbene-2-O-β-D-glucoside; Polygonum multiflorum; UPLC fingerprint; cluster analysis; principal components analysis

[Indexed for MEDLINE]

Conflict of interest statement

The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.

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