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Methods Mol Biol. 2017;1653:217-225. doi: 10.1007/978-1-4939-7225-8_15.

Investigating the Role of the Photorespiratory Pathway in Non-photosynthetic Tissues.

Author information

1
Max-Planck-Institute of Molecular Plant Physiology, Am M├╝hlenberg 1, 14476, Potsdam-Golm, Germany. fernie@mpimp-golm.mpg.de.
2
Plant Physiology Department, University of Rostock, Rostock, Germany.
3
Department of Plant Sciences, University of Oxford, Oxford, UK.

Abstract

Whilst photorespiration represents one of the dominant pathway fluxes in photosynthetic tissues there are hints from publically available gene expression data such as that housed in the bioarray resource (BAR; www.bar.utoronto.ca) that several of the constituent enzymes are present in roots and other heterotrophic tissues. Here we describe a protocol based on modification of the gaseous environment surrounding individual tissues of mutant and wild type Arabidopsis and evaluation of the consequences. This method could additionally easily be used for larger plants.

KEYWORDS:

Metabolite analysis; Photorespiratory mutants; Split-plant gas experiment

PMID:
28822136
DOI:
10.1007/978-1-4939-7225-8_15
[Indexed for MEDLINE]

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