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J Hosp Infect. 2018 Feb;98(2):155-160. doi: 10.1016/j.jhin.2017.08.009. Epub 2017 Aug 14.

Genomic characterization of a paediatric MRSA outbreak by next-generation sequencing.

Author information

1
Istituto Giannina Gaslini, Genova, Italy.
2
Istituto Giannina Gaslini, Genova, Italy. Electronic address: robertobiassoni@gaslini.org.

Abstract

INTRODUCTION:

Twelve strains of meticillin-resistant Staphylococcus aureus (MRSA) isolated during a suspected outbreak in a paediatric intensive care unit were analysed by whole-genome sequencing (WGS).

AIM:

To define the clonality of MRSA strains to a high discriminative power, and to evaluate the presence of genetic determinants responsible for antibiotic resistance and virulence.

RESULTS:

Ten out of 12 strains belonged to multi-locus sequence type ST2625, while the other two strains were ST8. Among the ST2625 strains, analysis based on 1126 genes showed that they were clonal, sharing more than 98.3% of allelic identities, and one strain was isolated from a healthcare worker. All ST2625 strains were characterized by the SCC-Mec cassette IVa, and resistoma analysis indicated correspondence between phenotypic and genotypic characteristics. The study of 63 genes associated with virulence was correlated with the pattern of clonality shown.

CONCLUSION:

This analysis confirmed the occurrence of an outbreak. As such, standard infection control measures were strictly enforced, and this led to prompt termination of the outbreak.

KEYWORDS:

MRSA; Paediatric nosocomial infections; Resistoma; Staphylococcus aureus; Toxoma; Whole-genome sequencing

PMID:
28818651
DOI:
10.1016/j.jhin.2017.08.009
[Indexed for MEDLINE]

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