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Thromb Haemost. 2017 Oct 5;117(10):1896-1907. doi: 10.1160/TH16-12-0904. Epub 2017 Aug 17.

Coagulation factor XII regulates inflammatory responses in human lungs.

Author information

1
Malgorzata Wygrecka, PhD, Department of Biochemistry, Faculty of Medicine,, Universities of Giessen and Marburg Lung Center, Friedrichstrasse 24, 35392 Giessen, Germany, Tel.: +49 641 99 47482, Fax: +49 641 99 47509, E-mail: malgorzata.wygrecka@innere.med.uni-giessen.de.

Abstract

Increased procoagulant activity in the alveolar compartment and uncontrolled inflammation are hallmarks of the acute respiratory distress syndrome (ARDS). Here, we investigated whether the contact phase system of coagulation is activated and may regulate inflammatory responses in human lungs. Components of the contact phase system were characterized in bronchoalveolar lavage fluids (BALF) from 54 ARDS patients and 43 controls, and their impact on cytokine/chemokine expression in human precision cut lung slices (PCLS) was assessed by a PCR array. Activation of the contact system, associated with high levels of coagulation factor XIIa (Hageman factor, FXIIa), plasma kallikrein and bradykinin, occurred rapidly in ARDS lungs after the onset of the disease and virtually normalized within one week from time of diagnosis. FXII levels in BALF were higher in ARDS non-survivors than survivors and were positively correlated with tumor necrosis factor (TNF)-α concentration. FXII induced the production and release of interleukin (IL)-8, IL-1β, IL-6, leukemia inhibitory factor (LIF), CXCL5 and TNF-α in human PCLS in a kallikrein-kinin-independent manner. In conclusion, accumulation of FXII in ARDS lungs may contribute to the release of pro-inflammatory mediators and is associated with clinical outcome. FXII inhibition may thus offer a novel and promising therapeutic approach to antagonize overwhelming inflammatory responses in ARDS lungs without interfering with vital haemostasis.

KEYWORDS:

Acute respiratory distress syndrome; coagulation factor XII; contact phase system

PMID:
28816340
DOI:
10.1160/TH16-12-0904
[Indexed for MEDLINE]

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