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Sci Rep. 2017 Aug 15;7(1):8150. doi: 10.1038/s41598-017-08327-w.

Plasma metabolomics profiling of maintenance hemodialysis based on capillary electrophoresis - time of flight mass spectrometry.

Liu S1, Wang L2,3,4, Hu C5,6, Huang X7, Liu H1, Xuan Q2,4, Lin X7, Peng X3, Lu X2,4, Chang M8, Xu G9,10.

Author information

1
Nephrology Department, Dalian Municipal Central Hospital, 826 Xinan Road, Dalian, 116033, China.
2
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China.
3
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China.
4
University of Chinese Academy of Sciences, Beijing, 100049, China.
5
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China. hucx@dicp.ac.cn.
6
University of Chinese Academy of Sciences, Beijing, 100049, China. hucx@dicp.ac.cn.
7
School of Computer Science & Technology, Dalian University of Technology, 116024, Dalian, China.
8
Nephrology Department, Dalian Municipal Central Hospital, 826 Xinan Road, Dalian, 116033, China. mingchang@yeah.net.
9
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, China. xugw@dicp.ac.cn.
10
University of Chinese Academy of Sciences, Beijing, 100049, China. xugw@dicp.ac.cn.

Abstract

Uremia has been a rapidly increasing health problem in China. Hemodialysis (HD) is the main renal replacement therapy for uremia. The results of large-scale clinical trials have shown that the HD pattern is crucial for long-term prognosis of maintenance hemodialysis (MHD) in uremic patients. Plasma metabolism is very important for revealing the biological insights linked to the therapeutic effects of the HD pattern on uremia. Alteration of plasma metabolites in uremic patients in response to HD therapy has been reported. However, HD-pattern-dependent changes in plasma metabolites remain poorly understood. To this end, a capillary electrophoresis-time of flight mass spectrometry (CE-TOF/MS)-based metabolomics method was performed to systemically study the differences between HD and high flux hemodialysis (HFD) on plasma metabolite changes in patients. Three hundred and one plasma samples from three independent human cohorts (i.e., healthy controls, patients with pre-HD/post-HD, and patients with pre-HFD/post-HFD) were used in this study. Metabolites significantly changed (p < 0.05) after a single HD or HFD process. However, 11 uremic retention solutes could be more efficiently removed by HFD. Our findings indicate that a CE-TOF/MS-based metabolomics approach is promising for providing novel insights into understanding the effects of different dialysis methods on metabolite alterations of uremia.

PMID:
28811533
PMCID:
PMC5557835
DOI:
10.1038/s41598-017-08327-w
[Indexed for MEDLINE]
Free PMC Article

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