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Methods Mol Biol. 2017;1656:119-129. doi: 10.1007/978-1-4939-7237-1_6.

RNA PAMPs as Molecular Tools for Evaluating RIG-I Function in Innate Immunity.

Author information

1
Department of Immunology, Center for Innate Immunity and Immune Disease, University of Washington School of Medicine, E383, 750 Republican Street, Seattle, WA, 98109, USA.
2
Department of Immunology, Center for Innate Immunity and Immune Disease, University of Washington School of Medicine, E383, 750 Republican Street, Seattle, WA, 98109, USA. mgale@uw.edu.

Abstract

Pathogen recognition receptors (PRR)s and their cognate pathogen-associated molecular pattern (PAMP) represent the basis of innate immune activation and immune response induction driven by the host-pathogen interaction that occurs during microbial infection in humans and other animals. For RNA virus infection such as hepatitis C virus (HCV) and others, specific motifs within viral RNA mark it as nonself and visible to the host as a PAMP through interaction with RIG-I-like receptors including retinoic inducible gene-I (RIG-I). Here, we present methods for producing and using HCV PAMP RNA as a molecular tool to study RIG-I and its signaling pathway, both in vitro and in vivo, in innate immune regulation.

KEYWORDS:

Innate immunity; PAMP; Pathogen recognition receptor; RNA; Rig-I

PMID:
28808965
DOI:
10.1007/978-1-4939-7237-1_6
[Indexed for MEDLINE]

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