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J Leukoc Biol. 2017 Nov;102(5):1261-1269. doi: 10.1189/jlb.4A0317-116R. Epub 2017 Aug 14.

Human mucosal-associated invariant T (MAIT) cells possess capacity for B cell help.

Author information

1
Division of Infectious Diseases, Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City, Utah, USA; and.
2
Division of Microbiology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.
3
Division of Infectious Diseases, Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City, Utah, USA; and daniel.leung@utah.edu.

Abstract

Mucosal-associated invariant T (MAIT) cells are an innate-like T cell subset, restricted by the nonclassic MHC class I-related protein MR1 and enriched at mucosal sites. Human studies have shown an association between MAIT cells and pathogen-specific antibody responses. In this study, we investigate the effect of human MAIT cells on B cells ex vivo. We found that supernatants from microbe- or cytokine-stimulated MAIT cells, when added to purified autologous B cells, increase frequencies of plasmablasts and promote IgA, IgG, and IgM production. We found effects to be mostly MR1-dependent and that the increases in plasmablasts are likely a result of increased differentiation from memory B cells. Furthermore, microbe-activated MAIT cell supernatant contains multiple cytokines known to stimulate B cells, including IL-6, -10, and -21. This study thus provides the first direct evidence of a newly identified role of MAIT cells in providing help to B cells.

KEYWORDS:

B cell help; MR-1; antibody; plasmablast

PMID:
28807929
PMCID:
PMC5636046
DOI:
10.1189/jlb.4A0317-116R
[Indexed for MEDLINE]
Free PMC Article

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