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J Invest Dermatol. 2017 Dec;137(12):2630-2638. doi: 10.1016/j.jid.2017.08.003. Epub 2017 Aug 12.

MicroRNA-132 with Therapeutic Potential in Chronic Wounds.

Author information

1
Unit of Dermatology and Venereology, Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
2
Department of Dermatology, The Second Hospital of Dalian Medical University, Dalian, China.
3
Department of Wound Regeneration, The Second Hospital of Dalian Medical University, Dalian, China.
4
Department of Endocrinology, Metabolism and Diabetes, Karolinska University Hospital, Stockholm, Sweden; Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
5
Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
6
Department of Reconstructive Plastic Surgery, Karolinska University Hospital, Stockholm, Sweden.
7
Unit of Dermatology and Venereology, Department of Medicine, Karolinska Institutet, Stockholm, Sweden; Unit of Dermatology and Venereology, Karolinska University Hospital, Stockholm, Sweden.
8
Department of Biosciences and Nutrition and Center for Innovative Medicine, Karolinska Institutet, Huddinge, Sweden.
9
Unit of Dermatology and Venereology, Department of Medicine, Karolinska Institutet, Stockholm, Sweden. Electronic address: ning.xu@ki.se.

Abstract

Chronic wounds represent a major and rising health and economic burden worldwide. There is a continued search toward more effective wound therapy. We found significantly reduced microRNA-132 (miR-132) expression in human diabetic ulcers compared with normal skin wounds and also in skin wounds of leptin receptor-deficient (db/db) diabetic mice compared with wild-type mice. Local replenishment of miR-132 in the wounds of db/db mice accelerated wound closure effectively, which was accompanied by increased proliferation of wound edge keratinocytes and reduced inflammation. The pro-healing effect of miR-132 was further supported by global transcriptome analysis, which showed that several inflammation-related signaling pathways (e.g., NF-κB, NOD-like receptor, toll-like receptor, and tumor necrosis factor signaling pathways) were the top ones regulated by miR-132 in vivo. Moreover, we topically applied liposome-formulated miR-132 mimics mixed with pluronic F-127 gel on human ex vivo skin wounds, which promoted re-epithelialization. Together, our study showed the therapeutic potential of miR-132 in chronic wounds, which warrants further evaluation in controlled clinical trials.

PMID:
28807666
DOI:
10.1016/j.jid.2017.08.003
[Indexed for MEDLINE]
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