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Iran J Basic Med Sci. 2017 Apr;20(4):451-456. doi: 10.22038/IJBMS.2017.8589.

Zinc oxide nanoparticle reduced biofilm formation and antigen 43 expressions in uropathogenic Escherichiacoli.

Author information

1
Department of Microbiology, Faculty of Medical Sciences, Golestan University of Medical Sciences, Gorgan, Iran.
2
Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran.

Abstract

OBJECTIVES:

This study aimed to investigate the effect of zinc oxide nanoparticles (ZnO-np) on biofilm formation and expression of the flu gene in uropathogenic Escherichia coli (UPEC) strains.

MATERIALS AND METHODS:

Minimum inhibitory concentration (MIC) of ZnO-np was determined by agar dilution method. The effect of MIC and sub-MIC concentrations of ZnO-np on biofilm formation were determined by microtiter plate assay. The expression level of the flu gene was assessed by Real-Time PCR assay.

RESULTS:

MIC and sub-MIC ZnO-np concentrations reduced biofilm formation by 50% and 33.4%, respectively. Sub-MIC ZnO-np concentration significantly reduced the flu gene expression in the UPEC isolates (P<0.0001).

CONCLUSION:

The sub-MIC concentration of ZnO-np reduces biofilm formation and flu gene expression in UPEC isolates. It is suggested to use nanoparticles for coating medical devices to prevent bacterial colonization.

KEYWORDS:

Biofilm; Urinary tract infection; Uropathogenic Escherichia -coli; Zinc oxide nanoparticle

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