Format

Send to

Choose Destination
Front Neural Circuits. 2017 Jul 28;11:52. doi: 10.3389/fncir.2017.00052. eCollection 2017.

Whole-Brain Mapping of the Inputs and Outputs of the Medial Part of the Olfactory Tubercle.

Zhang Z1,2,3, Zhang H4, Wen P2, Zhu X2, Wang L2,3, Liu Q2, Wang J2, He X2, Wang H2, Xu F2,3,5.

Author information

1
College of Life Science and Technology, Huazhong University of Science and TechnologyWuhan, China.
2
Center for Brain Science, Key Laboratory of Magnetic Resonance in Biological Systems and State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of SciencesWuhan, China.
3
Wuhan National Laboratory for OptoelectronicsWuhan, China.
4
College of Life Sciences, Wuhan UniversityWuhan, China.
5
Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of SciencesShanghai, China.

Abstract

The medial part of the olfactory tubercle (OT) is a brain structure located at the interface of the reward and olfactory system. It is closely related to pheromone-rewards, natural reinforcement, addiction and many other behaviors. However, the structure of the anatomic circuitry of the medial part of the OT is still unclear. In the present study, the medial part of the OT was found to be highly connected with a wide range of brain areas with the help of the pseudorabies virus tracing tool. In order to further investigate the detailed connections for specific neurons, another tracing tool - rabies virus was utilized for D1R-cre and D2R-cre mice. The D1R and D2R neurons in the medial part of the OT were both preferentially innervated by the olfactory areas, especially the piriform cortex, and both had similar direct input patterns. With the help of the adeno-associated virus labeling, it was found that the two subpopulations of neurons primarily innervate with the reward related brain regions, with slightly less axons projecting to the olfactory areas. Thus, the whole-brain input and output circuitry structures for specific types of neurons in the medial part of the OT were systematically investigated, and the results revealed many unique connecting features. This work could provide new insights for further study into the physiological functions of the medial part of the OT.

KEYWORDS:

D1R and D2R neurons; neuronal circuitry; the medial part of the OT; virus tracing tools; whole-brain mapping

PMID:
28804450
PMCID:
PMC5532451
DOI:
10.3389/fncir.2017.00052
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Frontiers Media SA Icon for PubMed Central
Loading ...
Support Center