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Vaccine. 2017 Sep 5;35(37):4966-4973. doi: 10.1016/j.vaccine.2017.07.075. Epub 2017 Aug 10.

In vitro and in vivo studies of deglycosylated chimeric porcine reproductive and respiratory syndrome virus as a vaccine candidate and its realistic revenue impact at commercial pig production level.

Author information

1
Animal Health Management Division, Ministry of Agriculture, Food and Rural Affairs, 94 Dasom2-ro, Government Complex-Sejong, Sejong-si 30110, Republic of Korea; Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
2
Division of Vaccine Research, Korea National Institute of Health, Korea Center for Disease Control and Prevention, Osong Health Technology Administration Complex, Osongsaengmueong2-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 28159, Republic of Korea.
3
Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
4
KBNP, INC., 254-18, Dugok-ri, Sinam, Yesan-si, Chungchungnam-do 32417, Republic of Korea.
5
Daesung Microbiological Labs. Co., Ltd., 5F, Soam Building, 208, Bangbae-ro, Seocho-gu, Seoul 06585, Republic of Korea.
6
Sungwoo Agricultural Co., 40-3 Hongnamseo-ro, 843 beon-gil, Gyeolseong-myeon, Hongseong-gun, Chungcheongnam-do 32210, Republic of Korea.
7
College of Veterinary Medicine, Midwestern University, 19555, North 59th Avenue, Glendale, AZ 85308, USA.
8
The Warren Alpert Medical School of Brown University, Providence, RI 02903, USA.
9
Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea; Veterinary Epidemiology Division, Animal and Plant Quarantine Agency, 177 Hyeoksin8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Republic of Korea. Electronic address: vet10@naver.com.
10
Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. Electronic address: virus@konkuk.ac.kr.

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in the swine industry worldwide. Vaccination is the most effective method to control the disease. In a previous study, a chimeric PRRSV named as K418 which had a genome composed of ORF 1 from the FL12 strain and ORF 2-7 from the Korean representative LMY strain was created. We constructed K418DM, K418 with deglycosylated glycoprotein 5 (GP5), to improve its humoral immunity. In the follow-up on in vivo and in vitro virological and serological tests, no back mutation in amino acids of GP5 associated with deglycosylation was shown after 9 passages on MARC-145 cells, whereas only one case of back mutation was detected after single passage in pig. In serological study, K418DM induced higher serum neutralization (SN) antibody and more limited viremia compared with those of K418 virus. In clinical trial and economic analysis, the K418DM elicited SN antibody titers and PRRSV-specific IgG over protection limit. From the economic viewpoint, there was statistically significant reduction in percentage of weak pigs. These results indicated that vaccination with the K418DM may provide enhanced protection for pigs in PRRS endemic situation and increase growth performance in commercial pig farms.

KEYWORDS:

Economic effect; GP5; K418DM; PRRSV (Porcine reproductive and respiratory syndrome virus); Vaccine

PMID:
28802752
DOI:
10.1016/j.vaccine.2017.07.075
[Indexed for MEDLINE]

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