Format

Send to

Choose Destination
J Photochem Photobiol B. 2017 Sep;174:229-234. doi: 10.1016/j.jphotobiol.2017.07.030. Epub 2017 Jul 29.

Fungicidal activity of copper-sputtered flexible surfaces under dark and actinic light against azole-resistant Candida albicans and Candida glabrata.

Author information

1
Group of Advanced Oxidation Procedures, Swiss Federal Institute of Technology, EPFL-SB- ISIC-GPAO, Station 6, CH-1015 Lausanne, Switzerland; Department of Fundamental Microbiology, University of Lausanne, Quartier Unil-Sorge, Biophore Building, CH-1015 Lausanne, Switzerland.
2
Group of Advanced Oxidation Procedures, Swiss Federal Institute of Technology, EPFL-SB- ISIC-GPAO, Station 6, CH-1015 Lausanne, Switzerland.
3
Department of Fundamental Microbiology, University of Lausanne, Quartier Unil-Sorge, Biophore Building, CH-1015 Lausanne, Switzerland. Electronic address: jose.entenza@unil.ch.
4
Department of Fundamental Microbiology, University of Lausanne, Quartier Unil-Sorge, Biophore Building, CH-1015 Lausanne, Switzerland.

Abstract

Candida spp. are able to survive on hospital surfaces and causes healthcare-associated infections (HCAIs). Since surface cleaning and disinfecting interventions are not totally effective to eliminate Candida spp., new approaches should be devised. Copper (Cu) has widely recognized antifungal activity and the use of Cu-sputtered surfaces has recently been proposed to curb the spread of HCAIs. Moreover, the activity of Cu under the action of actinic light remains underexplored. We investigated the antifungal activity of Cu-sputtered polyester surfaces (Cu-PES) against azole-resistant Candida albicans and Candida glabrata under dark and low intensity visible light irradiation (4.65mW/cm2). The surface properties of Cu-PES photocatalysts were characterized by diffuse reflectance spectroscopy (DRS) and X-ray fluorescence (XRF). Under dark, Cu-PES showed a fungicidal activity (≥3log10CFU reduction of the initial inoculum) against both C. albicans DSY296 and C. glabrata DSY565 leading to a reduction of the starting inoculum of 3.1 and 3.0log10CFU, respectively, within 60min of exposure. Under low intensity visible light irradiation, Cu-PES exhibited an accelerated fungicidal activity against both strains with a reduction of 3.0 and 3.4log10CFU, respectively, within 30min of exposure. This effect was likely due to the semiconductor Cu2O/CuO charge separation. The decrease in cell viability of the two Candida strains under dark and light conditions correlated with the progressive loss of membrane integrity. These results indicate that Cu-PES represent a promising strategy for decreasing the colonization of surfaces by yeasts and that actinic light can improve its self-disinfecting activity.

KEYWORDS:

Actinic light; Antifungal activity; Azole-resistant Candida spp.; Copper-sputtered polyester

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center