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Immunity. 2017 Aug 15;47(2):349-362.e5. doi: 10.1016/j.immuni.2017.07.019. Epub 2017 Aug 8.

T Cell Zone Resident Macrophages Silently Dispose of Apoptotic Cells in the Lymph Node.

Author information

1
Aix Marseille Univ, CNRS, INSERM, CIML, Marseille, France. Electronic address: baratin@ciml.univ-mrs.fr.
2
Aix Marseille Univ, CNRS, INSERM, CIML, Marseille, France.
3
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR7104, INSERM UMR964, Université de Strasbourg, F-67404 Illkirch, France.
4
Institute of Pathology, Charité Universitätsmedizin Berlin, 10117 Berlin, Germany.
5
Aix Marseille Univ, CNRS, INSERM, CIML, Marseille, France. Electronic address: bajenoff@ciml.univ-mrs.fr.

Abstract

In lymph nodes (LNs), dendritic cells (DCs) are thought to dispose of apoptotic cells, a function pertaining to macrophages in other tissues. We found that a population of CX3CR1+ MERTK+ cells located in the T cell zone of LNs, previously identified as DCs, are efferocytic macrophages. Lineage-tracing experiments and shield chimeras indicated that these T zone macrophages (TZM) are long-lived macrophages seeded in utero and slowly replaced by blood monocytes after birth. Imaging the LNs of mice in which TZM and DCs express different fluorescent proteins revealed that TZM-and not DCs-act as the only professional scavengers, clearing apoptotic cells in the LN T cell zone in a CX3CR1-dependent manner. Furthermore, similar to other macrophages, TZM appear inefficient in priming CD4 T cells. Thus, efferocytosis and T cell activation in the LN are uncoupled processes designated to macrophages and DCs, respectively, with implications to the maintenance of immune homeostasis.

KEYWORDS:

dendritic cell; efferocytosis; lymph node; macrophage

PMID:
28801233
DOI:
10.1016/j.immuni.2017.07.019
[Indexed for MEDLINE]
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