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Chembiochem. 2017 Oct 18;18(20):2000-2006. doi: 10.1002/cbic.201700425. Epub 2017 Sep 1.

Computation-Guided Design of a Stimulus-Responsive Multienzyme Supramolecular Assembly.

Yang L1,2, Dolan EM1,2, Tan SK2, Lin T2, Sontag ED3,2,4, Khare SD1,5,3,2.

Author information

1
Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.
2
Center for Integrative Proteomics Research, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.
3
Institute for Quantitative Biomedicine, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.
4
Department of Mathematics, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.
5
Computational Biology & Molecular Biophysics Program, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.

Abstract

The construction of stimulus-responsive supramolecular complexes of metabolic pathway enzymes, inspired by natural multienzyme assemblies (metabolons), provides an attractive avenue for efficient and spatiotemporally controllable one-pot biotransformations. We have constructed a phosphorylation- and optically responsive metabolon for the biodegradation of the environmental pollutant 1,2,3-trichloropropane.

KEYWORDS:

biodegradation; enzymes; photo-crosslinking; protein design; supramolecular enzyme assembly

PMID:
28799209
DOI:
10.1002/cbic.201700425
[Indexed for MEDLINE]

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