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Anal Bioanal Chem. 2018 Jan;410(3):933-941. doi: 10.1007/s00216-017-0548-7. Epub 2017 Aug 10.

Automated microfluidic devices integrating solid-phase extraction, fluorescent labeling, and microchip electrophoresis for preterm birth biomarker analysis.

Author information

1
Department of Chemistry and Biochemistry, Brigham Young University, C100 BNSN, Provo, UT, 84602-5700, USA.
2
Department of Chemistry and Biochemistry, Brigham Young University, C100 BNSN, Provo, UT, 84602-5700, USA. atw@byu.edu.

Abstract

We have developed multichannel integrated microfluidic devices for automated preconcentration, labeling, purification, and separation of preterm birth (PTB) biomarkers. We fabricated multilayer poly(dimethylsiloxane)-cyclic olefin copolymer (PDMS-COC) devices that perform solid-phase extraction (SPE) and microchip electrophoresis (μCE) for automated PTB biomarker analysis. The PDMS control layer had a peristaltic pump and pneumatic valves for flow control, while the PDMS fluidic layer had five input reservoirs connected to microchannels and a μCE system. The COC layers had a reversed-phase octyl methacrylate porous polymer monolith for SPE and fluorescent labeling of PTB biomarkers. We determined μCE conditions for two PTB biomarkers, ferritin (Fer) and corticotropin-releasing factor (CRF). We used these integrated microfluidic devices to preconcentrate and purify off-chip-labeled Fer and CRF in an automated fashion. Finally, we performed a fully automated on-chip analysis of unlabeled PTB biomarkers, involving SPE, labeling, and μCE separation with 1 h total analysis time. These integrated systems have strong potential to be combined with upstream immunoaffinity extraction, offering a compact sample-to-answer biomarker analysis platform. Graphical abstract Pressure-actuated integrated microfluidic devices have been developed for automated solid-phase extraction, fluorescent labeling, and microchip electrophoresis of preterm birth biomarkers.

KEYWORDS:

Capillary electrophoresis/electrophoresis; Clinical/biomedical analysis; Extraction (SFE|SPE|SPME); Microfluidics/microfabrication; Separations/instrumentation

PMID:
28799040
PMCID:
PMC5775915
DOI:
10.1007/s00216-017-0548-7
[Indexed for MEDLINE]
Free PMC Article

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