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Front Plant Sci. 2017 Jul 27;8:1333. doi: 10.3389/fpls.2017.01333. eCollection 2017.

Molecular Basis Underlying Leaf Variegation of a Moth Orchid Mutant (Phalaenopsis aphrodite subsp. formosana).

Author information

1
Kaohsiung District Agricultural Research and Extension StationPingtung, Taiwan.
2
Department of Biological Science and Technology, National Pingtung University of Science and TechnologyPingtung, Taiwan.
3
Department of Food Science and Nutrition, Meiho UniversityPingtung, Taiwan.
4
Department of Life Sciences and Research Center for Global Change Biology, National Chung Hsing UniversityTaichung, Taiwan.
5
Department of Life Science, National Taiwan Normal UniversityTaipei, Taiwan.
6
Department of Biological Sciences, National Sun Yat-sen UniversityKaohsiung, Taiwan.
7
Department of Biomedical Science and Environment Biology, Kaohsiung Medical UniversityKaohsiung, Taiwan.

Abstract

Leaf variegation is often the focus of plant breeding. Here, we studied a variegated mutant of Phalaenopsis aphrodite subsp. formosana, which is usually used as a parent of horticultural breeding, to understand its anatomic and genetic regulatory mechanisms in variegation. Chloroplasts with well-organized thylakoids and starch grains were found only in the mesophyll cells of green sectors but not of yellow sectors, confirming that the variegation belongs to the chlorophyll type. The two-dimensional electrophoresis and LC/MS/MS also reveal differential expressions of PsbP and PsbO between the green and yellow leaf sectors. Full-length cDNA sequencing revealed that mutant transcripts were caused by intron retention. When conditioning on the total RNA expression, we found that the functional transcript of PsbO and mutant transcript of PsbP are higher expressed in the yellow sector than in the green sector, suggesting that the post-transcriptional regulation of PsbO and PsbP differentiates the performance between green and yellow sectors. Because PsbP plays an important role in the stability of thylakoid folding, we suggest that the negative regulation of PsbP may inhibit thylakoid development in the yellow sectors. This causes chlorophyll deficiency in the yellow sectors and results in leaf variegation. We also provide evidence of the link of virus CymMV and the formation of variegation according to the differential expression of CymMV between green and yellow sectors.

KEYWORDS:

alternative polyadenylation; alternative splicing; chloroplast structure; differential expression protein; proteome analysis; variegation mutant

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