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J Clin Microbiol. 2017 Oct;55(10):3089-3103. doi: 10.1128/JCM.00881-17. Epub 2017 Aug 9.

Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic-Device-Related Infections Correlated with Patient Outcome.

Author information

1
AO Research Institute Davos, Davos, Switzerland.
2
Microbiology and Infectious Diseases, Institute of Life Science, Swansea University Medical School, Swansea, United Kingdom.
3
Department of Orthopedic and Trauma Surgery, University Hospital Basel, Basel, Switzerland.
4
Department of Trauma Surgery, Trauma Centre Murnau, Murnau, Germany.
5
The Milner Centre for Evolution, Department of Biology & Biochemistry, University of Bath, Bath, United Kingdom.
6
AO Research Institute Davos, Davos, Switzerland fintan.moriarty@aofoundation.org.

Abstract

Staphylococcus epidermidis has emerged as an important opportunistic pathogen causing orthopedic-device-related infections (ODRI). This study investigated the association of genome variation and phenotypic features of the infecting S. epidermidis isolate with the clinical outcome for the infected patient. S. epidermidis isolates were collected from 104 patients with ODRI. Their clinical outcomes were evaluated, after an average of 26 months, as either "cured" or "not cured." The isolates were tested for antibiotic susceptibility and biofilm formation. Whole-genome sequencing was performed on all isolates, and genomic variation was related to features associated with "cured" and "not cured." Strong biofilm formation and aminoglycoside resistance were associated with a "not-cured" outcome (P = 0.031 and P < 0.001, respectively). Based on gene-by-gene analysis, some accessory genes were more prevalent in isolates from the "not-cured" group. These included the biofilm-associated bhp gene, the antiseptic resistance qacA gene, the cassette chromosome recombinase-encoding genes ccrA and ccrB, and the IS256-like transposase gene. This study identifies biofilm formation and antibiotic resistance as associated with poor outcome in S. epidermidis ODRI. Whole-genome sequencing identified specific genes associated with a "not-cured" outcome that should be validated in future studies. (The study has been registered at ClinicalTrials.gov with identifier NCT02640937.).

KEYWORDS:

MRSE; Staphylococcus epidermidis; antibiotic resistance; genotype; orthopedic-device-related infections; phenotype; virulence factors

PMID:
28794175
PMCID:
PMC5625394
DOI:
10.1128/JCM.00881-17
[Indexed for MEDLINE]
Free PMC Article

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