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Cell Physiol Biochem. 2017;42(5):1945-1960. doi: 10.1159/000479835. Epub 2017 Aug 9.

Macrophage Overexpressing NGAL Ameliorated Kidney Fibrosis in the UUO Mice Model.

Author information

1
Experimental Nephrology, Department of Ciències Clíniques, Universitat de Barcelona, Institut d'Investigació biomèdica de Bellvitge (IDIBELL), Hospitalet de Llobregat, Barcelona, Spain.
2
Department of Ischemia and Inflammation, Institut d'lnvestigacions Biomèdiques de Barcelona (IIBB), Barcelona, Spain.
3
Hospital Universitari de Bellvitge, Hospitalet de Llobregat, Barcelona, Spain.

Abstract

BACKGROUND/AIMS:

Alternatively activated macrophages (AAM) have regenerative and anti-inflammatory characteristics. Here, we sought to evaluate whether AAM cell therapy reduces renal inflammation and fibrosis in the unilateral ureteral obstruction (UUO) mice model.

METHODS:

We stabilized macrophages by adenoviral vector NGAL (Neutrophil gelatinase-associated lipocalin-2) and infused them into UUO mice. To ascertain whether macrophages were capable of reaching the obstructed kidney, macrophages were stained and detected by in vivo cell tracking.

RESULTS:

We demonstrated that some infused macrophages reached the obstructed kidney and that infusion of macrophages overexpressing NGAL was associated with reduced kidney interstitial fibrosis and inflammation. This therapeutic effect was mainly associated with the phenotype and function preservation of the transferred macrophages isolated from the obstructed kidney Conclusions: Macrophage plasticity is a major hurdle for achieving macrophage therapy success in chronic nephropathies and could be overcome by transferring lipocalin-2.

KEYWORDS:

Alternatively activated macrophages; Chronic kidney disease; Macrophage plasticity

PMID:
28793288
DOI:
10.1159/000479835
[Indexed for MEDLINE]
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