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Radiat Environ Biophys. 2017 Nov;56(4):365-375. doi: 10.1007/s00411-017-0704-7. Epub 2017 Aug 8.

Abscopal effect of boron neutron capture therapy (BNCT): proof of principle in an experimental model of colon cancer.

Author information

1
Department of Radiobiology, Comisión Nacional de Energía Atómica (CNEA), Avenida General Paz 1499, B1650KNA San Martin, Provincia Buenos Aires, Argentina.
2
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina.
3
Department of Research and Production Reactors, Centro Atómico Ezeiza, Comisión Nacional de Energía Atómica (CNEA), Provincia Buenos Aires, Argentina.
4
Instituto de Oncología Ángel H. Roffo, Ciudad Autónoma de Buenos Aires, Argentina.
5
Department of Instrumentation and Control, Comisión Nacional de Energía Atómica (CNEA), Provincia Buenos Aires, Argentina.
6
Department of Boron Neutron Capture Therapy, Comisión Nacional de Energía Atómica (CNEA), Provincia Buenos Aires, Argentina.
7
Faculty of Exact and Natural Sciences, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.
8
Department of Radiobiology, Comisión Nacional de Energía Atómica (CNEA), Avenida General Paz 1499, B1650KNA San Martin, Provincia Buenos Aires, Argentina. schwint@cnea.gov.ar.
9
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina. schwint@cnea.gov.ar.

Abstract

The aim of the present study was to evaluate, for the first time, the abscopal effect of boron neutron capture therapy (BNCT). Twenty-six BDIX rats were inoculated subcutaneously with 1 × 106 DHD/K12/TRb syngeneic colon cancer cells in the right hind flank. Three weeks post-inoculation, the right leg of 12 rats bearing the tumor nodule was treated with BPA-BNCT (BPA-Boronophenylalanine) at the RA-3 nuclear reactor located in Buenos Aires, Argentina, at an absorbed dose of 7.5 Gy to skin as the dose-limiting tissue. The remaining group of 14 tumor-bearing rats were left untreated and used as control. Two weeks post-BNCT, 1 × 106 DHD/K12/TRb cells were injected subcutaneously in the contralateral left hind flank of each of the 26 BDIX rats. Tumor volume in both legs was measured weekly for 7 weeks to determine response to BNCT in the right leg and to assess a potential influence of BNCT in the right leg on tumor development in the left leg. Within the BNCT group, a statistically significant reduction was observed in contralateral left tumor volume in animals whose right leg tumor responded to BNCT (post-treatment/pre-treatment tumor volume <1) versus animals who failed to respond (post/pre ≥1), i.e., 13 ± 15 vs 271 ± 128 mm3. In addition, a statistically significant reduction in contralateral left leg tumor volume was observed in BNCT-responsive animals (post/pre <1) vs untreated animals, i.e., 13 ± 15 vs 254 ± 251 mm3. The present study performed in a simple animal model provides proof of principle that the positive response of a tumor to BNCT is capable of inducing an abscopal effect.

KEYWORDS:

Abscopal effect; BNCT; Boron neutron capture therapy; Boronophenylalanine (BPA); Nuclear reactor RA-3

PMID:
28791476
DOI:
10.1007/s00411-017-0704-7
[Indexed for MEDLINE]

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