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Sci Rep. 2017 Aug 8;7(1):7545. doi: 10.1038/s41598-017-06144-9.

Single Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses.

Author information

1
KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea.
2
Department of Mechanical and Industrial Engineering, College of Engineering, Northeastern University, Boston, MA, 02115, USA.
3
Gordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA.
4
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA, 02115, USA.
5
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA, 02115, USA. he.lee@neu.edu.
6
Department of Nano-Integrated Cogno-Mechatronics, Engineering, Pusan National University, Busan, South Korea. he.lee@neu.edu.

Abstract

Miniaturized microfluidic biosensors have recently been advanced for portable point-of-care diagnostics by integrating lab-on-a-chip technology and electrochemical analysis. However, the design of a small, integrated, and reliable biosensor for multiple and simultaneous electrochemical analyses in a single device remains a challenge. Here, we present a simultaneous microfluidic electrochemical biosensing system to detect multiple biomarkers of pulmonary hypertension diseases in a single device. The miniaturized biosensor, which is composed of five chambers, is precisely and individually controlled using in-house-built pneumatic microvalves to manipulate the flow pathway. Each chamber is connected to an electrochemical sensor designed to detect four different biomarkers plus a reference control. Our design allows for loading of multiple reagents for simultaneous analyses. On the basis of the developed microfluidic electrochemical sensor system, we successfully detected four well-defined pulmonary hypertension-associated biomarkers, namely, fibrinogen, adiponectin, low-density lipoprotein, and 8-isoprostane. This novel approach offers a new platform for a rapid, miniaturized, and sensitive diagnostic sensor in a single device for various human diseases.

PMID:
28790334
PMCID:
PMC5548735
DOI:
10.1038/s41598-017-06144-9
[Indexed for MEDLINE]
Free PMC Article

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