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Sci Rep. 2017 Aug 8;7(1):7545. doi: 10.1038/s41598-017-06144-9.

Single Microfluidic Electrochemical Sensor System for Simultaneous Multi-Pulmonary Hypertension Biomarker Analyses.

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KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea.
Department of Mechanical and Industrial Engineering, College of Engineering, Northeastern University, Boston, MA, 02115, USA.
Gordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA.
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA, 02115, USA.
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA, 02115, USA.
Department of Nano-Integrated Cogno-Mechatronics, Engineering, Pusan National University, Busan, South Korea.


Miniaturized microfluidic biosensors have recently been advanced for portable point-of-care diagnostics by integrating lab-on-a-chip technology and electrochemical analysis. However, the design of a small, integrated, and reliable biosensor for multiple and simultaneous electrochemical analyses in a single device remains a challenge. Here, we present a simultaneous microfluidic electrochemical biosensing system to detect multiple biomarkers of pulmonary hypertension diseases in a single device. The miniaturized biosensor, which is composed of five chambers, is precisely and individually controlled using in-house-built pneumatic microvalves to manipulate the flow pathway. Each chamber is connected to an electrochemical sensor designed to detect four different biomarkers plus a reference control. Our design allows for loading of multiple reagents for simultaneous analyses. On the basis of the developed microfluidic electrochemical sensor system, we successfully detected four well-defined pulmonary hypertension-associated biomarkers, namely, fibrinogen, adiponectin, low-density lipoprotein, and 8-isoprostane. This novel approach offers a new platform for a rapid, miniaturized, and sensitive diagnostic sensor in a single device for various human diseases.

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