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Nat Commun. 2017 Aug 9;8(1):220. doi: 10.1038/s41467-017-00220-4.

Plasmonic silver nanoshells for drug and metabolite detection.

Author information

1
School of Biomedical Engineering, Children's Hospital of Shanghai, and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China.
2
Department of Chemistry, Shanghai University, Shanghai, 200444, People's Republic of China.
3
Institute of Biophysics Key Laboratory of Interdisciplinary Research, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
4
School of Biomedical Engineering, Children's Hospital of Shanghai, and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China. rubinchen@126.com.
5
School of Biomedical Engineering, Children's Hospital of Shanghai, and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China. k.qian@sjtu.edu.cn.

Abstract

In-vitro metabolite and drug detection rely on designed materials-based analytical platforms, which are universally used in biomedical research and clinical practice. However, metabolic analysis in bio-samples needs tedious sample preparation, due to the sample complexity and low molecular abundance. A further challenge is to construct diagnostic tools. Herein, we developed a platform using silver nanoshells. We synthesized SiO2@Ag with tunable shell structures by multi-cycled silver mirror reactions. Optimized nanoshells achieved direct laser desorption/ionization mass spectrometry in 0.5 μL of bio-fluids. We applied these nanoshells for disease diagnosis and therapeutic evaluation. We identified patients with postoperative brain infection through daily monitoring and glucose quantitation in cerebrospinal fluid. We measured drug distribution in blood and cerebrospinal fluid systems and validated the function of blood-brain/cerebrospinal fluid-barriers for pharmacokinetics. Our work sheds light on the design of materials for advanced metabolic analysis and precision diagnostics.Preparation of samples for diagnosis can affect the detection of biomarkers and metabolites. Here, the authors use a silver nanoparticle plasmonics approach for the detection of biomarkers in patients as well as investigate the distribution of drugs in serum and cerebral spinal fluid.

PMID:
28790311
PMCID:
PMC5548796
DOI:
10.1038/s41467-017-00220-4
[Indexed for MEDLINE]
Free PMC Article

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